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Direct conversion of human fibroblasts into neural progenitors via the use of transcription factors highly enriched in human ESC-derived neural progenitors

Stem Cell Reports 2016 Dec 7. pii: S2213-6711(16)30268-5. doi: 10.1016/j.[Epub ahead of print]

http://www.sciencedirect.com/science/article/pii/S2213671116302685

Early human embryonic stem cell (hESC)-derived neural populations consist of various embryonic neural progenitors (ENPs) with broad neural developmental propensity. Here, we sought to directly convert human somatic cells into ENP-like phenotypes using hESC-ENP-enriched neural transcription factors (TFs). We demonstrated that induced ENP could be efficiently converted from human fibroblasts using two TF combinations. The iENPs exhibit cellular and molecular characteristics resembling hESC-ENPs and can give rise to astrocytes, oligodendrocytes, and functional neuronal subtypes of the central and peripheral nervous system. Nevertheless, our analyses further revealed that these two iENP populations differ in terms of their proliferation ability and neuronal propensity. Finally, we demonstrated that the iENPs can be induced from fibroblasts from patients with Huntington's disease and Alzheimer's disease, and the diseased iENPs and their neuronal derivatives recapitulated the hallmark pathological features of the diseases. Collectively, our results point toward a promising strategy for generating iENPs from somatic cells for disease modeling and future clinical intervention.

HESC-NP enriched neural factor (HESC-NP NF): a new paradigm for direct conversion of human somatic cells into neural tissues and cells

 

Hou et. al. Stem cell Reports, 2016

 

 


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115台北市南港區研究院路二段128號
Tel: 02-27899515
Fax: 02-27858059
icob@gate.sinica.edu.tw
Copyright © ICOB 2013. All rights reserved. 最佳瀏覽網頁方式請用最新版IE11或其他瀏覽器 /瀏覽人數:914268--
 瀏覽人數:914268