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Genetic Reprogramming of Positional Memory in a Regenerating Appendage

出版日期:2019-12-02

First published: Current Biology, November 27, 2019

https://www.cell.com/current-biology/fulltext/S0960-9822(19)31378-8

Certain vertebrates such as salamanders and zebrafish are able to regenerate complex tissues (e.g., limbs and fins) with remarkable fidelity. However, how positional information of the missing structure is recalled by appendage stump cells has puzzled researchers for centuries. Here, we report that sizing information for adult zebrafish tailfins is encoded within proliferating blastema cells during a critical period of regeneration. Using a chemical mutagenesis screen, we identified a temperature-sensitive allele of the gene encoding DNA polymerase alpha subunit 2 (pola2) that disrupts fin regeneration in zebrafish. Temperature shift assays revealed a 48-h window of regeneration, during which positional identities could be disrupted in pola2 mutants, leading to regeneration of miniaturized appendages. These fins retained memory of the new size in subsequent rounds of amputation and regeneration. Similar effects were observed upon transient genetic or pharmacological disruption of progenitor cell proliferation after plucking of zebrafish scales or head or tail amputation in amphioxus and annelids. Our results provide evidence that positional information in regenerating tissues is not hardwired but malleable, based on regulatory mechanisms that appear to be evolutionarily conserved across distantly related phyla.

 

"「再生」也可能長歪? 科學界首度證實:動物的再生記憶可被改寫"  -《中研院訊》第1704期

https://newsletter.sinica.edu.tw/20191128f03/

 

"研究:蠑螈.斑馬魚再生記憶可被改寫"  -《台視新聞 TTV NEWS》

https://www.youtube.com/watch?v=0cuvt6scnT4

 


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115台北市南港區研究院路二段128號
Tel: 02-27899515
Fax: 02-27858059
icob@gate.sinica.edu.tw
Copyright © ICOB 2013. All rights reserved. 最佳瀏覽網頁方式請用最新版IE11或其他瀏覽器 /瀏覽人數:1531891--
 瀏覽人數:1531891