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姓名:周雅惠

職稱:副研究員

電話: 02-2789-9540

專長:Developmental Neurobiology,
Neuronal circuitry,
Insect Behavior

Link:

研究人員 / 簡介

  2019~, Associate Research Fellow, Institute of Cellular and Organismic Biology, Academia Sinica, Taiwan
  2011-2019, Assistant Research Fellow, Institute of Cellular and Organismic Biology, Academia Sinica, Taiwan
  2004-2011, Postdoctoral Research Fellow, Department of Biology, Stanford University, USA
  2003-2004, Postdoctoral Research Fellow, Institute of Molecular Biology, Academia Sinica, Taiwan
  2003, PhD. Institute of Molecular Biology, Academia Sinica and Institute of Life Science, National Defense Medical Center, Taiwan

神經迴路及行為實驗室

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    莊子曰 :「子非我,安知我不知魚之樂 ? 」—莊子 〈秋水〉 

     

    如同莊子與惠子的魚樂之辯,當面對同一顆蘋果,我們也無從得知自己聞到的蘋果味道是否與旁人聞到的完全一樣。事實是,雖然每個人的大腦都經由相同的發育機轉而架構、成熟,當面對相同的環境刺激,每個個體接收訊息的程度並不會完全相同,而這種差異最終會反映在我們對周遭環境的判讀及行為反應。我們實驗室即對神經差異性 (Neuronal variability) 的形成及其對個體行為的影響有興趣。

    果蠅利用與哺乳動物相似的嗅覺神經系統來分辨環境中的氣味分子 (Odorant),如其天敵、食物、配偶的氣味,以決定下一刻要作何反應。果蠅的嗅神經細胞 (Olfactory receptor neuron; ORN) 一旦接收環境中的氣味分子,會將訊息傳遞到腦中的嗅葉  (Antennal lobe; AL)。在此,二級神經元 (Projection neuron; PN) 會接收訊息並將其傳遞到不同的腦區域中心,如 mushroom body (MB) 及 lateral horn (LH) 去做進一步的訊息整合及判讀。然而在嗅球周圍還有二群區域中介神經元 (Local interneuron; LN),他們在氣味分子訊息被傳遞到二級神經元之前,即先將這些訊息整合、變化及轉譯 (圖一)。這些區域中介神經元有的只涵蓋單側的嗅 (圖二),有的則是同時涵蓋二側的嗅 (圖三)。

    果蠅的嗅覺神經系統相較於哺乳類動物簡單,但卻可以接收並處理龐大、複雜的訊息而決定其多樣、精細的行為反應。因此我們希望利用此神經迴路來探討神經差異性的源起及其分子機制。我們之前發現,果蠅嗅覺神經系統的區域中介神經元具有相當複雜的多樣性 (Diversity) 及差異性 (Variability),但對於這些細胞如何在發育過程中及個體成熟後達到其多樣性及差異性一無所知。因此我們希望經由系統性地研究區域中介神經元的發育、其如何選擇與特定的嗅覺神經元及二級神經元形成獨特的連結、以及其差異性對個體行為的影響,來瞭解不同個體大腦中神經迴路的差異性是如何造成的,以及其如何影響我們對外界環境的判讀。

    目前我們實驗室進行中的研究包括 : (1)系統性的研究不同類型區域中介神經元的發育,並經由篩選找出調控其發育的分子及機制; (2)利用我們已發展出的標定單一特定區域中介神經元系統、來探討區域中介神經元差異性的起源及其對果蠅嗅覺行為的影響; (3)利用我們收集到的大量行為實驗數據,建立數學模擬系統、以暸解區域中介神經元如何影響嗅覺訊息在神經迴路的變化及傳遞。

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

  • 姓名職稱電話Email備註
    周雅惠助研究員02-27899540
    yhchou@gate.sinica.edu.twAug 2011~
    蔡國鼎博士後02-27899509
    kuoting.tsai@gmail.comAug 2014~
    楊己任研究助理02-27899509
    ermae@gate.sinica.edu.twJune 2014~
    劉南甫研究助理 (大專生)02-27899509
    nonfood666@gate.sinica.edu.twSep. 2016~ (Sep. 2011~June 2014)
    張顥馨研究助理 02-27899509
    s0170543@gate.sinica.edu.twAug. 2016~
    王桂枝助理02-27899509
    --Jan 2012~
    黃予辰助理02-27899509
    --Apr 2018 ~
    周星賢醫師02-27899509
    b85401053@ntu.edu.twAug 2011~
    前實驗室成員CURRENT POSITION
    林子揚研究助理09/2011~05/2013
    Graduate Student/Dresden International PhD Program/Germany
    楊士又研究助理08/2012~12/2013
    施秉宏大專生06/2012~02/2014
    Master Student/National Tsing Hua U.
    李承翰大專生07/2013~06/2014
    Undergraduate Student/National Taiwan U. School of Medicine
    李冠緯大專生03/2012~06/2014
    Graduate Student, Department of Physics, National Taiwan University
    陳盈君研究助理10/2011~06/2014
    台灣東洋藥品股份有限公司/專利工程師
    陳郁唐研究助理03/2014~05/2016
    Graduate Student/UT Southwestern Medical Center, USA
    吳廷翰研究助理04/2013~08/2016
    Graduate Student/TIGP-MCB, Academia Sinica
    林欣儒研究助理04/2013~08/2016
    Postgraduate Student/Dept Computer Science, National Tsing Hua U.
    李岑佑研究助理 08/2016-08/2017
    Graduate Student/ Graduate Training Centre of Neuroscience, Tübingen Neural Information Processing Program, University of Tübingen, Germany
    林詩涵研究助理08/2012-12/2017
    新旭生技股份有限公司/實驗室經理
    梁書暘研究助理 01/2018-02/2018
    Research Assistant/Institute of Molecular Biology, Academia Sinica
    張凱翔研究助理 07/2016-03/2018
    PGS technician, Cooper Genomics, USA
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    SELECTED PUBLICATIONS

    (2019)

    1. Tsai, K.T., Liou, N.F. and Chou, Y.H.* (2019). Quantitatively analyzing morphological phenotypes of neurons and neuropils through three-dimensional reconstruction of Drosophila brains. Bio-101. e1010290.  DOI: 10.21769/BioProtoc.10101290. (in Chinese) (In press)

    2. Chang, H. H. and Chou, Y. H.* (2019). Labeling single cells by Mosaic analysis with a repressible maker (MARCM) in Drosophila nervous system. Bio-101. e1010289. DOI 10.21769/BioProtoc.10101289. (in Chinese) (In press)

    3. Yang, C.J., Tsai, K.T., Liou, N.F., Chou, Y.H.* (2019) Interneuron diversity: toward a better understanding of interneuron development in the olfactory system. J. Experimental Neuroscience. 13, 1-5. (Invited review). DOI:10.1177/1179069519826056

    4. Chen, Y.J.#, Chang, H.H.#, Lin, S.H.#, Lin, T.Y., Wu, T.H., Lin, H.J., Liou, N.F., Yang, C.J., Chen, Y.T., Chang, K.H., Li,C.Y., Chou, Y.H.* (2019) Differential efficacy of genetically swapping GAL4. J. Neurogenetics (Epub ahead of print). DOI:10.1080/01677063.2018.1564289
     
    (2018)
    5. Liou, N.F.#, Lin, S.H.#, Chen, Y.J.#, Tsai, K.T.&, Yang, C.J.&, Lin, T.Y., Wu, T.H., Lin, H.J., Chen, Y.T., Gohl, D.M., Silies, M., Chou, Y.H.* (2018) Diverse populations of local interneurons integrate into the Drosophila adult olfactory circuit. Nature Communications 9, 2232. DOI:10.1038/s41467-018-04675-x
    Recommended by F1000Prime. Hiesinger P:F1000Prime, 10.3410 (Dec. 2018) 10.3410/f.733424303.793554440
     
    6. Tsai, K.T., Hu, C.K., Li, K.W., Hwang, W.L., Chou, Y.H.* (2018) Circuit variability interacts with excitatory-inhibitory diversity of interneurons to regulate network encoding capacity. Scientific Reports 8, 8027. DOI:10.1038/s41598-018-26286-8
     
    (2017)
    7. Hsia, E.Y.C., Zhang, Y., Tran, H.S., Lim, A., Chou, Y.H., Lan, G., Beachy, P.A., Zheng, X. (2017) Hedgehog mediate degradation of Ihog adhesion peoteins modulates cell segragation in Drosophila wing imaginal discs. Nature Communications  8, 1275. DOI:10.1038/s41467-017-01364-z
     
    8. Wu, B., Li J., Chou, Y.H., Luginbuhl D., and Luo L. (2017) Fibroblast growth factor signaling instructs ensheathing glia warpping of Drosophila olfactory glomeruli. PNAS 114, 7505-7512. DOI:10.1073/pnas.1706533114
     
    (2011)
    9. Sweeney, L.B., Chou, Y.H.#, Wu, Z.#, Joo W.#, Komiyama, T., Potter, C.J., Kolodkin, A., Garcia, K.C., and Luo, L. (2011) Secreted Semaphorins from degenerating larval ORN axons direct adult projection neuron dendrite targeting. Neuron 72, 734-747. DOI:10.1016/j.neuron.2011.09.026
    # equal authorship
     
    (2000-2010)
    10. Chou, Y.H., Zheng, X., Beachy, P.A. and Luo, L. (2010) Patterning axon targeting of olfactory receptor neurons by coupled Hedgehog signaling at two distinct steps. Cell 142, 954-966. DOI:10.1016/j.cell.2010.08.015
    Featured in Nat. Rev. Neurosci, Research Highlights. (Nov. 2010) DOI:10.1038/nrn2940
     
    11. Chou, Y.H.#, Spletter, M.L.#, Yaksi, E.#, Leong, J.C.S., Wilson, R.I. and Luo, L. (2010) Diversity and wiring variability of olfactory local interneurons in the Drosophila antennal lobe. Nature Neuroscience 13, 439-449. DOI:10.1038/nn.2489
    # equal authorship
    Featured on the cover
     
    12. Sweeney, L.B., Couto, A.#Chou, Y.H.#, Berdnik, D., Dickson, B.J., Luo, L., and Komiyama, T. (2007). Temporal target restriction of olfactory receptor neurons by Semaphorin-1a/PlexinA-mediated axon-axon interactions. Neuron 53, 185-200. DOI:10.1016/j.neuron.2006.12.022
    # equal authorship
    Preview by Greg J. Bashaw DOI:10.1016/j.neuron.2007.01.002
     
    13. Chou, Y.H. and Chien, C.T. (2002). Scabrous controls ommatidial rotation in the Drosophila compound eye. Developmental Cell 3, 839-850. https://doi.org/10.1016/S1534-5807(02)00362-3
     
    (Before 2000)
    14. Hsu, S.L., Chen, M.C., Chou, Y.H., Hwang, G.Y., and Yin, S.C. (1999). Induction of P21CIP1/Waf1 and activation of p34cdc2 involved in retinoic acid-induced apoptosis in human hepatoma Hep3B cells. Experimental Cell Resesrach 248, 87-96. DOI:10.1006/excr.1999.4397
     
    15. Hsu, S.L., Chou, Y.H., Yin, S.C., and Liu, J.Y. (1998). Differential effects of phorbol ester on growth and protein kinase C isoenzyme regulation in human hepatoma Hep3B cells. Biochemical Journal 333, 57-64. http://www.biochemj.org/content/333/1/57.long
     
    16. Chou, Y.H. and Yung, B.Y. M. (1995). Cell cycle phase-dependent changes of localization and oligomerization states of nucleophosmin/B23. Biochemical Biophysical Research Communications 217, 313-325. DOI:10.1006/bbrc.1995.2779

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    115台北市南港區研究院路二段128號
    Tel: 02-27899515
    Fax: 02-27858059
    icob@gate.sinica.edu.tw
    Copyright © ICOB 2013. All rights reserved. 最佳瀏覽網頁方式請用最新版IE11或其他瀏覽器 /瀏覽人數:1492526--
     瀏覽人數:1492526