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姓名:游宏祥

職稱:助研究員

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專長:Neural Development, Neuroscience

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研究人員 / 簡介
  2011-present, Assistant Research Fellow, Institute of Cellular and Organismic Biology, Academia Sinica, Taiwan.
  2008-2011, Research Specialist, Janelia Farm Research Campus, Howard Hughes Medical Institute, USA.
  2005-2008, Postdoctoral Fellow, Department of Neurobiology, University of Massachusetts Medical School, USA.
  2000-2005, Postdoctoral Fellow, Division of Basic Sciences, Fred Hutchinson Cancer Research Center, USA.
  2000, Ph.D., Department of Neuroscience, Johns Hopkins University School of Medicine, USA.

 

游宏祥之神經科學 實驗室

  • 動物利用散佈在周遭環境的味道來尋找食物,挑選伴侶和躲避敵人。假如錯把敵人的氣味誤認為食物及伴侶的味道,將對動物造成無可彌補的災難。因此發育過程中,建構正確的嗅覺神經網路,對動物的生存扮演著十分重要的角色。在嗅覺神經網路內,周邊神經系統上同類的嗅覺感覺神經元(olfactory sensory neuron)偵測到嗅覺分子後,將嗅覺訊號經傳入大腦嗅葉(antennal lobe)上的單一嗅球(glomerulus),再由嗅覺投射神經元(olfactory projection neuron)傳到大腦其它區域(圖一),包含蘑菇形體(mushroom body)及側角組織(lateral horn)。我的實驗室主要是研究嗅覺投射神經元在發育過程中是如何建構嗅覺神經網路,以及這些嗅覺投射神經元在嗅覺相關行為的功能。

    我們利用兩種追蹤細胞族譜的染色技術[MARCM (mosaic analysis with a repressible cell marker) and twin-spot MARCM (圖二)]來研究嗅覺投射神經元在發育過程中是如何建構嗅覺神經網路。我們最近的研究,發現小分子核糖核酸-34(microRNA-34;miR-34)參與γ類型蘑菇形體神經元的大範圍的軸突修剪,而此軸突修剪對建構果蠅成蟲的正確嗅覺神經網路,扮演著十分重要的角色(圖三)。另外,我們的研究也發現,一個穿膜蛋白(Semaphorin-1a;Sema-1a)可避免嗅覺投射神經元樹突入侵特定的大腦嗅葉區域,以達成嗅覺投射神經元樹突連結到正確的大腦嗅葉區域,這對建構果蠅成蟲有功能的嗅覺神經網路,有著不可或缺的角色(圖四)。我們已經建立了使用鈣離子影像來觀測嗅覺投射神經元是如何對嗅覺感覺神經元的刺激作出反應。我們將使用一系列的行為實驗(包含性行為;圖五)來瞭解嗅覺投射神經元在嗅覺神經網路中的功能。我們的研究將會對動物如何從球投射神經元建構嗅覺神經網路,接收及整合嗅覺訊息到執行與嗅覺神經網路相關的行為有重要的貢獻,進而對古諺”食色性也”及” 飽暖思淫慾”解謎

     
     

     

     
     
     
     
     
     

     

  • 姓名職稱電話Email備註
    游宏祥助研究員
    賴彥瑋博班生
    璩劭宇碩班生
    古凱元研究助理
  • Publications:
    15. Shen, H.-C., Chu, S.-Y., Hsu, T.-C., Wang, C.-H., Lin, I.-Y. and Yu, H.-H#. (2017). Semaphorin-1a Prevents Drosophila Olfactory Projection Neuron Dendrites from Mis-targeting into Select Antennal Lobe Regions. PLoS Genet. 13(4): e1006751.  (corresponding author)
    14. Shen, H.-C., Hsu, T.-C., Chung, P.-C., and Yu, H.-H#. (2017). Cell Lineage Analyses and Gene Function Studies Using Twin-Spot MARCM. J. Vis. Exp. 121: doi: 10.3791/55278. (# corresponding author)
    13. Lai, Y.-W., Chu, S.-Y., Wei, J.-Y., Cheng, C.-Y., Li, J.-C., Chen, P.-L., Chen, C.-H.# and Yu, H.-H.# (2016). Drosophila microRNA-34 Impairs Axon Pruning of Mushroom Body γ Neurons by Downregulating the Expression of Ecdysone Receptor. Sci. Rep. 6: 39141.  (# co-corresponding authors)
    12.  Shen, H.-C*., Wei, J.-Y.*, Chu, S.-Y.*, Chung, P.-C.*, Hsu, T.-C.* and Yu, H.-H.# (2016). Morphogenetic Studies of the Drosophila DA1 Ventral Olfactory Projection Neuron. PLoS ONE 11(5): e0155384. (* co-first author; # corresponding author)
    11. Yang, J. S.*, Awasaki, T.*, Yu, H.-H.*, He, Y., Ding, P., Kao, J.-C. and Lee, T. (2013). Diverse neuronal lineages make stereotyped contributions to the Drosophila locomotor control center, the central complex. J. Comp. Neurol. 521, 2645-2662. (* co-first author)
    10. Yu, H.-H.*, Awasaki, T.*, Schroeder, M. D., Long, F., Yang, J. S., He, Y., Ding, P., Kao, J.-C., Wu, G. Y., Peng, H. and Lee, T. (2013). Clonal development and organization of the adult Drosophila central brain. Curr. Biol. 23, 633-643. (* co-first author)
    9. Lin, S., Kao, C.-F., Yu, H.-H., Huang, Y. and Lee, T. (2012). Lineage analysis of Drosophila lateral antennal lobe neurons reveals notch-dependent binary temporal fate decisions. PLoS Biol. 10, e1001425.
    8. Kao, C.-F., Yu, H.-H., He, Y., Kao, J.-C. and Lee, T. (2012). Hierarchical deployment of factors regulating temporal fate in a diverse neuronal lineage of the Drosophila central brain. Neuron 73, 677-684.
    7. Yu, H.-H.*, Kao, C.-F*., He, Y., Ding, P., Kao, J.-C. and Lee, T. (2010). A Complete Developmental Sequence of a Drosophila Neuronal Lineage as Revealed by Twin-Spot MARCM. PLoS Biol. 8, e1000461. (* co-first author)
    6. Lin, S.*, Lai, S.-L.*, Yu, H.-H.*, Chihara, T., Luo, L. and Lee, T. (2010). Lineage-specific Effects of Notch/Numb Signaling in Postembryonic Development of the Drosophila Brain. Development 137, 43-51. (* co-first author)
    5. Yu, H.-H., Chen, C.-H., Shi, L., Huang, Y.-L. and Lee, T. (2009). Twin-Spot MARCM to reveal developmental origin and identity of neurons. Nature Neurosci. 12, 947-953.
    4. Yu, H.-H.*, Yang, J. S.*, Wang, J., Huang, Y. and Lee, T. (2009). Endodomain diversity in Drosophila Dscam and its roles in neuronal morphogenesis. J. Neurosci. 29, 1904-1914. (* co-first author)
    3. Yu, H.-H. and Lee T. (2007) Neuronal Temporal Identity in Post-embryonic Drosophila Brain. Trends Neurosci. 30, 520-526.
    2. Shi, L.*, Yu, H.-H.*, Yang, J. S.*, and Lee, T. (2007). Specific Drosophila Dscam Juxtamembrane Variants Control Dendritic Elaboration and Axonal Arborization. J. Neurosci. 27, 6723-6728. (* co-first author)
    1. Soba, P., Zhu, S., Emoto, K., Younger, S., Yang, S. J., Yu, H.-H., Lee, T., Jan, L. Y., and Jan, Y. N. (2007). Drosophila sensory neurons require Dscam for dendritic self-avoidance and proper dendritic field organization. Neuron 54, 403-416.

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    115台北市南港區研究院路二段128號
    Tel: 02-27899515
    Fax: 02-27858059
    icob@gate.sinica.edu.tw
    Copyright © ICOB 2013. All rights reserved. 最佳瀏覽網頁方式請用最新版IE11或其他瀏覽器 /瀏覽人數:942600--
     瀏覽人數:942600