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姓名:郭紘志

職稱:副研究員

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專長:Stem Cell Biology
Molecular Embryology

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 研究人員 / 簡介

2013- Associate Professor,Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan
2013- Associate Professor,Institute of Biotechnology, National Taiwan Ocean University, Keelung, Taiwan
2013- Associate Research Fellow,Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan
2004-2013, Assistant Research Scientist,Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan
2002-2004, Staff Scientist, Oregon National Primate Research Center, Oregon Health Sciences, University, USA

2000-2002,

Postdoctoral Research, Associate Division of Reproductive Sciences, Oregon National Primate Research Center, USA

2000,

Ph.D. Reproductive Genetics, King’s College London, University of London, UK

 
 

 

郭紘志老師實驗室

  • 全能性幹細胞有兩種,一種是由發育中的胚胎所建立而成的胚胎幹細胞,另外一種是由體細胞再編程而建立的誘導性多能幹細胞。全能性幹細胞具有在體外或體內分化成各種細胞的能力,藉此所得分化的細胞可提供做為更新修復受傷或帶有病徵細胞的來源,亦可作為藥物篩選的平台。探討全能性幹細胞內調控自我更新與分化之間的平衡機制對於其應用是相當重要的。然而,雖然全能性幹細胞內的轉錄調控機制日益被了解,轉錄後機制如何去影響細胞的全能性維持與早期譜系分化仍相當未知。轉錄後修飾常常與非編碼RNA相關,而我們最近的研究發現反式剪接RNA在調控全能性幹細胞的全能性有嶄新的機制。為了探討非編碼RNA在細胞與分子的功能,我們整合全系統偵測方式、全能性幹細胞平台與體外分化的實驗。藉此綜合的方法,我們將能探索轉錄後修飾如何調控細胞分化命運以及變異的RNA如何導致人類疾病之謎。

    我們實驗室的另外一個研究主軸是探討轉錄因子如何調控全能性幹細胞形成神經外胚層如大腦與脊隨的網絡機制。我們已經成功建立許多體外神經分化的平台,利用這些平台,我們得以探討重要的轉錄因子,如LHX2與SOX2在全能性幹細胞往神經方向分化所扮演的角色。除此之外,我們也利用全能性幹細胞體外形成的立體大腦結構來探討調控早期大腦皮層分化的分子機轉。另外,我們也利用帶有疾病的人類誘導型全能性幹細胞及其分化的神經前驅細胞作為體外研究平台,以期找到新的臨床治療方式。

    我們實驗室的研究主要著重於:

    • 環狀RNA與反式剪接RNA在維持全能性、再編程與調控分化的功能
    • 人類全能性細胞進行大腦皮層分化的分子機轉
    • 利用人類誘導型全能性幹細胞以其分化的神經前驅細胞做為平台探討神經退化性疾病
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    郭紘志副研究員
  • 著作目錄

    1. Yu CY, Liu HJ, (Kuo HC*), Chuang TJ*. (2014). Is an observed non-co-linear RNA product spliced in trans, in cis, or just in vitro? NUCLEIC ACIDS RESEARCH. (In press)
    2. Lin YI, Chiu FL, Yeang CH, ChenHF, Chuang CY, Yang SY, Hou PS, Sintupisut N, Ho HN, (Kuo HC)*, Lin KI*. Suppression of the SOX2 neural effector gene by PRDM1 promotes human germ cell fate in embryonic stem cells. STEM CELL REPORTS (Cell Press), 2(2):189-204. 2014-01
    3. Wu CS, Yu CY, Chuang CY, Hsiao M, Kao CF, (Kuo HC*), Chuang TJ*. Integrative transcriptome sequencing identifies trans-splicing events with important roles in human embryonic stem cell pluripotency. GENOME RESEARCH. 24(1):25-36., 2014-01 (Highlighed by Nature Reviews Genetics)
    4. Hou PS, Chuang CY, Kao CF, Chou SJ, Stone L, Ho HN, Chien Cl, (Kuo HC)*. LHX2 regulates the neural differentiation of human embryonic stem cells via transcriptional modulation of PAX6 and CER1. NUCLEIC ACIDS RESEARCH. 41(16):7753-7770, 2013-09
    5. Huang HP, Chuang CY, and (Kuo HC)*. Induced pluripotent stem cell technology for disease modeling and drug screening with emphasis on lysosomal storage diseases. STEM CELL RESEARCH AND THERAPY. 3( 34), 2012-08. 
    6. Chuang CY, Lin KI, Hsiao M, Chen HF, Huang YH, Lin SP, Ho HN, (Kuo HC)*. Meiotic competent human germ cell-like cells derived from human embryonic stem cells induced by BMP4/WNT3a signaling and OCT4/EpCAM selection. JOURNAL OF BIOLOGICAL CHEMISTRY. 287(18), 14389-14401, 2012-04.
    7. Huang HP, Chen PH, Hwu WL, Chuang CY, Chien YH, Lee S, Chien CL, Li LT, Chen HF, Ho HN, Chen CH and (Kuo HC)*. Pompe disease induced pluripotent stem cells for pathogenesis modeling, drug testing and disease marker identification. HUMAN MOLECULAR GENETICS. 20(24), 4851-4864, 2011-12. 
    8. Huang HP, Chen PH, Yu CY, Chuang CY, Stone L, Hsiao WC, Li CL, Tsai SC, Chen KV, Chen HF, Ho HN, and (Kuo HC)*. Epithelial Cell Adhesion Molecule Complex Proteins Promote Transcription Factor-mediated Pluripotency Reprogramming. JOURNAL OF BIOLOGICAL CHEMISTRY. 286(38), 33520-33532, 2011-09. 
    9. Chen HF, Chuang CY, Lee WC, Huang HP, Wu HC, Ho HN, Chen YJ, and (Kuo HC)*. Surface marker epithelial cell adhesion molecule and E-cadherin facilitate the identification and selection of induced pluripotent stem cells . Stem Cell Reviews and Reports. 7(3), 722-735, 2011-09.
    10. Huang HP, Yu CY, Chen HF, Chen PH, Chuang CY, Lin SJ, Huang ST, Chan WH, Ueng TH, Ho HN, and (Kuo HC)*. Factors from human embryonic stem cell-derived fibroblast-like cells promote topology-dependent hepatic differentiation in primate embryonic stem cells and human induced pluripotent stem cells . JOURNAL OF BIOLOGICAL CHEMISTRY. 285(43), 33510-33519, 2010-10.

    Link to the complete list of publications


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    icob@gate.sinica.edu.tw  Copyright © ICOB 2013. All rights reserved. 最佳瀏覽網頁方式請用最新版IE11或其他瀏覽器 -- 瀏覽人數:914342
    115台北市南港區研究院路二段128號
    Tel: 02-27899515
    Fax: 02-27858059
    icob@gate.sinica.edu.tw
    Copyright © ICOB 2013. All rights reserved. 最佳瀏覽網頁方式請用最新版IE11或其他瀏覽器 /瀏覽人數:914342--
     瀏覽人數:914342