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姓名:傅琪鈺

職稱:助研究員

電話: 02-2789-9565

專長:3D electron microscopy
cellular structural biology
structural biology

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 研究人員 / 簡介 

2008-2013 Postdoctoral Fellow, Dept. of Integrative Structural and Computational Biology, The Scripps Research Institute
2007-2008 Postdoctoral Fellow, Dept. of Microbiology, University of Alabama at Birmingham
2001-2006 Ph.D. in Biochemistry and Molecular Genetics, University of Alabama at Birmingham

 

傅琪鈺老師實驗室

  • 我們感興趣粒線體結構和網絡動態是如何調控。我們使用3D電子顯微鏡方法來研究細胞和分子結構生物的調控機制。粒線體執行各種細胞過程,例如新陳代謝,鈣穩態,脂質合成和凋亡。它們的功能障礙與多種疾病有關,包括衰老,神經退化性疾病和癌症。粒線體具有獨特的雙膜結構與其功能息息相關。粒線體形態和網絡會因應細胞生理和細胞器功能進行動態重塑。我們的目標是闡明健康和神經退化性疾病中驅動粒線體形態和網絡動態重塑的分子和細胞機制。我們通過多學科方法解決這一問題,我們應用電子斷層掃描以揭示3D超微結構,使用相關連光學和電子顯微鏡(CLEM)來追踪細胞結構上的分子,並應用冷凍電顯和冷凍斷層掃描來研究關鍵分子的結構。我們旨在解構健康和神經退化性疾病中粒線體的結構和功能相關性。

     

  • 姓名職稱電話Email備註
    傅琪鈺助研究員02-2789-9565
    fuchiyu@gate.sinica.edu.tw
    1. Jiang, Y.F., H.L. Lin, L.J. Wang, T. Hsu, and C.Y. Fu* (2020). "Coordinated organization of mitochondrial lamellar cristae and gain of COX function during mitochondrial maturation in Drosophila." Mol Biol Cell. 31:18-26
    2. Jiang, Y. F., H. L. Lin; Fu, C. Y. * (2017). "3D mitochondrial ultrastructure of Drosophila indirect flight muscle revealed by serial-section electron tomography." J Vis Exp. 2017 Dec19: (130). doi:10.3791/56567.
    3. Tang, Y. L., I. J. Liu, P. C. Li, C. Y. Chiu, C. Y. Lin, C. H. Huang, Y. H. Chen, C. Y. Fu, D. Y. Chao, C. C. King and H. C. Wu (2017). "Generation and Characterization of Antinonstructural Protein 1 Monoclonal Antibodies and Development of Diagnostics for Dengue Virus Serotype 2." Am J Trop Med Hyg 97(4): 1049-1061.
    4. Jiang YF, Lin SS, Chen JM, Tsai HZ, Hsieh TS, Fu CY * (2017). “Electron tomographic analysis reveals ultrastructural features of mitochondrial cristae architecture which reflect energetic state and aging.” Sci Rep. 2017 Mar 30;7:45474
    5. Hou, S. C., H. S. Chen, H. W. Lin, W. T. Chao, Y. S. Chen, C. Y. Fu, C. M. Yu, K. F. Huang, A. H. Wang and A. S. Yang (2016). "High throughput cytotoxicity screening of anti-HER2 immunotoxins conjugated with antibody fragments from phage-displayed synthetic antibody libraries." Sci Rep. 6: 31878.
    6. Wang, K., C. Y. Fu, C. E. Catalano, P. E. Prevelige, P. C. Doerschuk and J. E. Johnson (2016). "Detecting asymmetry in the presence of symmetry with maximum likelihood three-dimensional reconstructions of viruses from electron microscope images." Iet Image Processing 10(8): 624-629.
    7. Veesler, D., M. G. Campbell, A. Cheng, C. Y. Fu, Z. Murez, J. E. Johnson, C. S. Potter and B. Carragher (2013). "Maximizing the potential of electron cryomicroscopy data collected using direct detectors." J Struct Biol 184(2): 193-202.
    8.  Veesler, D., T. S. Ng, A. K. Sendamarai, B. J. Eilers, C. M. Lawrence, S. M. Lok, M. J. Young, J. E. Johnson and C. Y. Fu * (2013). "Atomic structure of the 75 MDa extremophile Sulfolobus turreted icosahedral virus determined by CryoEM and X-ray crystallography." Proc Natl Acad Sci U S A 110(14): 5504-5509.
    9.  Fu, C. Y. and J. E. Johnson (2012). "Structure and cell biology of archaeal virus STIV." Curr Opin Virol 2(2): 122-127.
    10.  Macheboeuf, P., C. Buffalo, C. Y. Fu, A. S. Zinkernagel, J. N. Cole, J. E. Johnson, V. Nizet and P. Ghosh (2011). "Streptococcal M1 protein constructs a pathological host fibrinogen network." Nature 472(7341): 64-68.
    11. Huang, R. K., N. F. Steinmetz, C. Y. Fu, M. Manchester and J. E. Johnson (2011). "Transferrin-mediated targeting of bacteriophage HK97 nanoparticles into tumor cells." Nanomedicine (Lond) 6(1): 55-68.
    12.  Fu, C. Y. and J. E. Johnson (2011). "Viral life cycles captured in three-dimensions with electron microscopy tomography." Curr Opin Virol 1(2): 125-133.
    13.  Wang, K., C. Y. Fu, R. Khayat, P. C. Doerschuk and J. E. Johnson (2011). "In vivo virus structures: simultaneous classification, resolution enhancement, and noise reduction in whole-cell electron tomography." J Struct Biol 174(3): 425-433.
    14. Khayat, R., C. Y. Fu, A. C. Ortmann, M. J. Young and J. E. Johnson (2010). "The architecture and chemical stability of the archaeal Sulfolobus turreted icosahedral virus." J Virol 84(18): 9575-9583.
    15. Gertsman, I., C. Y. Fu, R. Huang, E. A. Komives and J. E. Johnson (2010). "Critical salt bridges guide capsid assembly, stability, and maturation behavior in bacteriophage HK97." Mol Cell Proteomics 9(8): 1752-1763.
    16. Fu, C. Y., K. Wang, L. Gan, J. Lanman, R. Khayat, M. J. Young, G. J. Jensen, P. C. Doerschuk and J. E. Johnson (2010). "In vivo assembly of an archaeal virus studied with whole-cell electron cryotomography." Structure 18(12): 1579-1586.
    17. Fu, C. Y., C. Uetrecht, S. Kang, M. C. Morais, A. J. Heck, M. R. Walter and P. E. Prevelige, Jr. (2010). "A docking model based on mass spectrometric and biochemical data describes phage packaging motor incorporation." Mol Cell Proteomics 9(8): 1764-1773.
    18.  Fu, C. Y. and P. E. Prevelige, Jr. (2009). "In vitro incorporation of the phage Phi29 connector complex." Virology 394(1): 149-153.
    19.  Poliakov, A., E. van Duijn, G. Lander, C. Y. Fu, J. E. Johnson, P. E. Prevelige, Jr. and A. J. Heck (2007). "Macromolecular mass spectrometry and electron microscopy as complementary tools for investigation of the heterogeneity of bacteriophage portal assemblies." J Struct Biol 157(2): 371-383.
    20. Fu, C. Y., M. C. Morais, A. J. Battisti, M. G. Rossmann and P. E. Prevelige, Jr. (2007). "Molecular dissection of o29 scaffolding protein function in an in vitro assembly system." J Mol Biol 366(4): 1161-1173.
    21. Fu, C. Y. and P. E. Prevelige, Jr. (2006). "Dynamic motions of free and bound O29 scaffolding protein identified by hydrogen deuterium exchange mass spectrometry." Protein Sci 15(4): 731-743. 

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    icob@gate.sinica.edu.tw  Copyright © ICOB 2013. All rights reserved. 最佳瀏覽網頁方式請用最新版IE11或其他瀏覽器 -- 瀏覽人數:1544362
    115台北市南港區研究院路二段128號
    Tel: 02-27899515
    Fax: 02-27858059
    icob@gate.sinica.edu.tw
    Copyright © ICOB 2013. All rights reserved. 最佳瀏覽網頁方式請用最新版IE11或其他瀏覽器 /瀏覽人數:1544362--
     瀏覽人數:1544362