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姓名:曾庸哲

職稱:助研究員

電話: 03-9880-544 ext.14

專長:Marine ecophysiology,
Evolutionary and comparative physiology,
Systeomics

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  2016/08- Assistant Research Fellow, Institute of Cellular and Organismic Biology, Academia Sinica, Taiwan
  2015/02-2016/08  Assistant Professor, Department of Life Science, National Taiwan Normal University, Taiwan
  2012/02-2015/01 Project-Appointed Assistant Professor, Department of Life Science, National Taiwan Normal University, Taiwan
  2011/07-2012/10

Distinguished Postdoctoral Fellow, Institute of Cellular and Organismic Biology , Academia Sinica, Taiwan

  2010/03-2011/08

Visiting Postdoctoral Scholar, Department of Biological Oceanography, Leibniz-Institute of Marine Sciences (IFM-GEOMAR), Germany

  2009/07-2011/06

Distinguished Postdoctoral Fellow, Institute of Cellular and Organismic Biology , Academia Sinica, Taiwan

  2009/03-2009/06

Regular Postdoctoral Fellow, Institute of Cellular and Organismic Biology , Academia Sinica, Taiwan

  2008/04-2008/10

 Postdoctoral Scholar, Department of Polar and Marine Research, Alfred Wegener Institute (AWI), Germany

  2008/04 Ph.D. Institute of Zoology, National Taiwan University, Taiwan

曾庸哲老師實驗室

  • 研究方向

         本實驗室致力於探討海洋動物面臨氣候變遷(海洋酸化及溫度變化)與極端環境因子波動衝擊下,海洋生物如何進行有效地進行生理調節機制以適應環境。本團隊運用次世代基因體學、代謝體學、行為表徵、以及表觀遺傳等研究方法,結合臺灣四面環海的絕佳海洋生物學研究優勢,以臺灣海域的頭足類動物(軟絲、花枝、章魚)、硬骨魚(吳郭魚、青鱂魚)、甲殼類動物(烏龜怪方蟹、大閘蟹)、以及棘皮類動物(海膽、陽隧足)為研究物種,進行綜合性比較生理學研究。以求突破過往海洋生物研究的知識與傳統,準確且深入地探討海洋動物生態學表徵背後的生理學意義。

    1. 海洋動物面臨海洋酸化及暖化的衝擊以及跨世代表觀遺傳適應機制

         海水表層的酸化情形往往伴隨著暖化現象的產生,可能會對於在此海域區段棲息的動物個體造成自小分子到大整體全面性的系統生理影響。而在此棲息的狹溫性動物在同時面臨高溫高碳酸刺激擾動下的生理衝擊,至今仍鮮為人知。

           本實驗室利用生物外表型模組觀測、即時生理參數檢測、次世代高速定序分析、行為表徵、表觀遺傳、以及基因表現相分析等研究方法,以全方位Eco – Evo - Devo-(生態 - 演化 - 發育)的生物網絡思維,研究海洋環境酸鹼值及溫度波動對於海洋運動型生物如頭足類動物(萊氏擬烏賊及章魚)、甲殼類動物(烏龜怪方蟹)、與硬骨魚類(青鱂魚)胚胎及成體的生理衝擊影響。

     2. 烏龜怪方蟹耐酸耐毒機制的細胞生理學研究

         深海熱泉支持著獨特的生態系統,其中包含了從微生物到脊椎動物豐富的生物多樣性。為了要在如此極端的環境生存,生物發展出特殊的形態和生理機制,以適應高溫、高硫、低氧、高二氧化碳和低酸鹼值的棲地。台灣北方的龜山島的淺海熱泉即是這類生物的代表棲地。龜山島熱泉的是一個高溫(76-116℃)、極度酸化(pH 1.5-2.5)的海底熱泉系統;此外,此處的排水含有大量的硫化物。在如此特殊的環境中,只有少數物種可以生存。例如在噴口附近的烏龜怪方蟹(Xenograpsus testudinatus),是唯一生存於此的後生動物。
        對甲殼類動物而言,鰓是參與細胞外離子與酸鹼平衡的主要器官,鰓表面的離子調節上皮所具有的離子主動/被動運輸機制在演化則相當的保守。在本團隊最近的研究中,我們找到烏龜怪方蟹主要的酸價與硫的排除通道。與脊椎動物相似,烏龜怪方蟹的鰓表皮細胞上有眾多的膜通道蛋白,這些通道幫浦在怪方蟹所有的鰓對上都有存在,並維持了烏龜怪方蟹的酸鹼平衡功能。此外,龜山島熱泉對生物的生理挑戰不僅是酸鹼調節,同時也包括了如何克服環境硫化氫的毒性。我們認為,在熱泉系統中的甲殼類應該具有比其他物種更好的控制硫化物與酸鹼恆定性。此外,日本九州鹿兒島南方海域也存在著與龜山島相似的淺海熱泉,在鹿兒島的熱泉生態系也發現與烏龜怪方蟹相似的種類。然而,此海域環境酸鹼值卻明顯低於龜山島海域。藉由比較在這兩個不同環境下生活的怪方蟹是否有不同的適應機制,可望了解酸鹼或硫化物調控的次主動運輸幫浦蛋白的演化關係。酸鹼與離子調節機制對甲殼類來說,不僅是一個物種演化的現象,在物種與生態的永續維持也至關重要。

    3. 生動物耐寒性狀的基因型、外表型及系統生物學研究

           低溫緊迫或酸化波動已知會使誘發水生動物複雜的基因相變化及生化反應,並在一些重要器官強化粒線體的活性以提高代謝機制。然而粒線體提升呼吸作用的同時可能會導致生物體的傷害,包括活性氧化物質(ROS)的形成累積、粒線體內膜氫離子的溢漏增加、以及脂質過氧化反應的產生。其中粒線體ROS過量累積已知會造成細胞面臨氧化壓力。因此,我們透過了解水生動物適應性代謝調節、轉錄體與代謝體資料庫建構、以及行為表徵的模式建立,綜合探討極端環境溫度變動下,水生動物個體內生理調節的變化,並進行細胞抗氧化相關機制的觀察。這些發現對於往後進一步研究並發展出應用於水生動物養殖產業的實際可行的策略,提供了重要的素材。

  • 姓名職稱電話Email備註
    曾庸哲助研究員+886-(0)3-9880544CC14
    yctseng@gate.sinica.edu.tw
    關寶龍研究助理+886-(0)3-9880544CC31
    jio47123456@yahoo.com.hk
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    rw6526@gmail.com
    周佩璇研究助理+886-(0)3-9880544CC24
    sherry821030@yahoo.com.tw
    林孟緯研究助理+886-(0)3-9880544CC24
    wayne51106@gmail.com
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    ockyf0814@gmail.com
    王敏貞博士班研究生+886-(0)3-9880544CC24
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    陳怡安 碩士班研究生+886-(0)3-9880544CC24
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    wayne51106@gmail.com
    許維恩大學部研習生+886-(0)3-9880544CC31
    hsuweien666@gmail.com
  • (A) Book Chapter
      Marian Y. Hu and Tseng. Y. C.# "Acid–Base Regulation and Ammonia Excretion in Cephalopods: An Ontogenetic Overview." Acid-Base Balance and Nitrogen Excretion in Invertebrates. Springer International Publishing, 2017. (pp. 275-298). (Corresponding author)
     
    (B) Published
     
    1. Shao, Y. T., Chang, S. Y., Chang, H. Y., Tseng, Y. C., Shao, K. T. (2018). Largescale mullet (Planiliza macrolepis) can recover from thermal pollution-induced malformations. PLoS ONE 13(11): e0208005.
    2. Narita, Y., Tsutiya, A., Nakano, Y., Ashitomi, M., Sato, K., Hosono, K., Kaneko, T., Chen, R. D., Lee, J. R., Tseng, T. C., Hwang, P. P., Otani-Kaneko, R. (2018). Androgen induced cellular proliferation, neurogenesis, and generation of GnRH3 neurons in the brain of mature female Mozambique tilapia. Sci Rep. 8, 16855.
    3. Shiao, J. C., Shirai, K., Tanaka, K., Takahata, N., Sano, Y., Hsiao, S. Y., Lee, D. C., Tseng, Y. C. (2018). Assimilation of nitrogen and carbon isotopes from fish diets to otoliths as measured by nanoscale secondary ion mass spectrometry. Rapid Commun Mass Spectrom, 32, 1250-1256, 2018.
    4. Hu, M. Y., Tseng, Y. C., Su, Y. H., Lein, E., Lee, J. H., Dupont, S., Stumpp, M. Variability in larval gut pH regulation defines sensitivity to ocean acidification in six species of the Ambulacraria superphylum. Proc R Soc Lond B Biol Sci, 287, 20171066, 2017.
    5. Wei, C. A., Lin, T. H., Che, R. D., Tseng, Y. C., Shao, Y. T. The effects of continuously acoustical stress on cortisol in milkfish (Chanos chanos). Gen Comp Endocrinol, 257, 227-234, 2017.
    6. Hu, M. Y., Sung, P. H., Guh, Y. J., Lee, J. R., Hwang, P. P., Weihrauch, D., Tseng, Y. C.# Perfused gills reveal fundamental principles of pH regulation and ammonia homeostasis in the cephalopod Octopus vulgaris. Front Physiol, 8, 162, 2017.
    7. Weaver, R., Hill, G. E., Kuan, P. L., Tseng, Y. C.# Copper exposure reduces production of red carotenoids in a marine copepod. Ecol Indic, 70, 393-400, 2016.
    8. Hu, M. Y., Michael, K., Kreiss, C. M., Stumpp, M., Dupont, S, Tseng, Y. C.#, Lucassen, M. Temperature modulates the effects of ocean acidification on intestinal ion transport in Atlantic cod, Gadus morhua. Front Physiol, 7, 198, 2016.
    9. Hu, M. Y., Guh, Y. J., Shao, Y. T., Kuan, P. L., Chen, G. L., Lee, J. R., Jeng, M. S., Tseng, Y. C.#. Strong ion regulatory abilities enable the crab Xenograpsus testudinatus to inhabit highly acidified marine vent systems. Front Physiol 7, 14, 2016.
    10.  Wang, Y. F., Yan, J. J., Tseng, Y. C. , Chen, R. D., Hwang, P. P. Molecular physiology of an extra-renal Cl- uptake mechanism for body fluid Cl- homeostasis. Int J Biol Sci 11(10), 1190-1203, 2015.
    11. Hu, M. Y., Hwang, P. P., Tseng, Y. C.# Recent advances in understanding trans-epithelial acid-base regulation and excretion mechanisms in cephalopods. Tissue Barriers 3(4), e1064196.
    12. Stumpp, M., Hu, M. Y., Tseng, Y. C., Guh, Y. J., Chen, Y. C., Su, Y. H., Yu, J. K., Hwang, P. P. Evolution of extreme pH in bilaterian digestive systems inferred from gastric alkalization mechanisms in basal deuterostomes. Sci Rep 5, 10421, 2015.
    13. Furukawa, F., Tseng, Y. C., Liu, S. T., Chou, Y. L., Lin, C. C., Sung, P. H., Uchida, K., Lin, L. Y., Hwang, P. P. Induction of phosphoenolpyruvate carboxykinase (PEPCK) during acute acidosis and its role in acid secretion by V-ATPase-expressing ionocytes. Int J Biol Sci 16(2), 712-725, 2015.
    14. Shao, Y. T., Tseng, Y. C.#, Chang, C. H., Yan, H. Y, Hwang, P. P., Borg, B. GnRH mRNA levels in male three-spined sticklebacks, Gasterosteus aculeatus, under different reproductive conditions. Comp Biochem Physiol A Mol Integr Physiol 180, 6-17, 2015.
    15. Hu, M. Y., Guh, Y. J., Stumpp, M., Lee, J. R., Chen, R. D., Sung, P. H., Chen, Y. C., Hwang, P. P. and Tseng, Y. C.#. Branchial NH4+-dependent acid-base transport mechanisms and energy metabolism of squid (Sepioteuthis lessoniana) affected by seawater acidification. Front Zool 11, 55, 2014.
    16. Tseng, Y. C., Liu, S. T., Hu, M. Y., Chen, R. D., Lee, J. R., and Hwang, P. P. Brain functioning under acute hypothermic stress supported by dynamic monocarboxylate utilization and transport in ectothermic fish. Front Zool 11, 53, 2014.
    17. Hsu, H. H., Lin, L. Y., Tseng, Y. C., Horng, J. L., and Hwang, P. P. A new model for fish ion regulation: Identification of ionocytes in freshwater- and seawater-acclimated medaka (Oryzias latipes). Cell Tissue Res 357(1), 225-243, 2014.
    18. Guh, Y. J., Tseng, Y. C., Yang, C. Y., and Hwang, P. P. Endothelin-1 regulates H+-ATPase-dependent transepithelial H+ secretion in zebrafish. Endocrinology 155(5), 1728-1737, 2014.
    19. Lee, L. C., Wang, Y. T., Wu., Y. R., Soong, B. W., Tseng, Y. C., Chen, C. M., Lee, G. J. Downregulation of proteins involved in the endoplasmic reticulum stress response and Nrf2-ARE signaling in lymphoblastoid cells of spinocerebellar ataxia type 17. J Neural Transm 121(6), 301-610, 2014.
    20. Chen, C. M., Wu, C. H., Hsieh, C. H., Lin, C. H., Chen, I. C., Chen, Y. C., Lee, L. C., Lee, C. M., Tseng, Y. C., Lee, G. J., and Wu, Y. R. HTRA2 variations in Taiwanese Parkinson’s disease. J Neural Transm 121(5), 491-198, 2014.
    21. Hu, M. Y., Lee, J. R., Lin, L. Y., Shih, T. H., Stumpp,M., Lee, M. F., Hwang, P. P. and Tseng, Y. C.#. Development in a naturally acidified environment: Na+/H+-exchanger 3-based proton secretion leads to CO2 tolerance in cephalopod embryos. Front Zool 10(1), 51, 2013.
    22. Tseng, Y. C., Hu, M. Y., Stumpp, M., Lin, L. Y., Melzner, F., and Hwang, P. P. CO2-driven seawater acidification differentially affects development and molecular plasticity along life history of fish (Oryzias latipes). Comp Biochem Physiol A Mol Integr Physiol 165(2), 119-130, 2013.
    23. Tseng, Y. C., Kao, Z. J., Liu, S. T., Chen, R. D., and Hwang, P. P. Spatial expression and functional flexibility of monocarboxylate transporter isoforms in the zebrafish brain. Comp Biochem Physiol A Mol Integr Physiol 165(2), 106-118, 2013.
    24. Shao, Y. T., Tseng, Y. C., Trombley, S., Hwang, P. P., Schmitz, M., and Borg, B. Schistocephalus solidus infections increase gonadotropins and gonadotropin releasing hormone (GnRH3) mRNA levels in the three-spined stickleback, Gasterosteus aculeatus. Parasitol Int 61(3), 470-474, 2012.
    25. Hu, M. Y.*, Tseng, Y. C.*, Lin, L. Y., Chen, P. Y., Charmantier-Daures, M., Hwang, P. P., and Melzner, F. New insights into ion regulation of cephalopod molluscs: a role of epidermal ionocytes in acid-base regulation during embryogenesis. Am J Physiol Regul Integr Comp Physiol 301(6), R1700-R1709, 2011. (* Equal Contribution)
    26. Tseng, Y. C., Chen, R. D., Lucassen, M., Schmidt, M. M., Abele, D., and Hwang, P. P. Exploring uncoupling proteins and anti-oxidative stress mechanisms under acute cold exposure in brains of fish. PLoS ONE 6(3), e18180, 2011.
    27. Hu, M. Y., Tseng, Y. C., Stumpp, M., Gutowska, M. A., Kiko, R., Lucassen, M., and Melzner, F. Elevated seawater pCO2 differentially affects branchial acid-base transporters over the course of development in the cephalopod Sepia officinalis. Am J Physiol Regul Integr Comp Physiol 300(5), R1100-R1114, 2011.
    28. Tseng, Y. C., Lee, J. R., Lee, S. J., and Hwang, P. P. Functional analysis of glucose transporter 1a, -6, and -13.1 expressed in epithelial cells. Am J Physiol Regul Integr Comp Physiol 300(2), R321-R329, 2011.
    29. Lafont, A. G., Wang, Y. F., Chen, G. D., Liao, B. K., Tseng, Y. C., Hwang, C. J., and Hwang, P. P. Involvement of calcitonin and its specific receptor in the control of calcium regulating-genes and calcium homeostasis in zebrafish (Danio rerio). J Bone Miner Res 26(5), 1072-1083, 2011.
    30. Cruz, S., Tseng, Y. C., Kaiya, H., and Hwang, P. P. Ghrelin affects carbohydrate-glycogen metabolism via insulin inhibition and glucagon stimulation in the zebrafish (Danio rerio) brain. Comp Biochem Physiol A Mol Integr Physiol 156(2), 190-200, 2010.
    31. Tseng, Y. C., Chen, R. D., Lee, J. R., Liu, S. T., Lee, S. J., and Hwang, P. P. Specific expression and regulation of glucose transporters in zebrafish ionocytes. Am J Physiol Regul Integr Comp Physiol 297(2), R275-R290, 2009. (a congratulation letter from the editor)
    32. Wang, Y. F., Tseng, Y. C., Yan, J. J., Hiroi, J., and Hwang, P. P. Role of SLC12A10.2, a Na-Cl cotransporter-like protein, in a Cl uptake mechanism in zebrafish (Danio rerio). Am J Physiol Regul Integr Comp Physiol 296(5), R1650-R1660, 2009.
    33. Tseng, D. Y., Chou, M. Y., Tseng, Y. C., Hsiao, C. D., Huang, C. J., Kaneko, T., and Hwang, P. P. Effects of Stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo. Am J Physiol Regul Integr Comp Physiol 296(3), R549-R557, 2009.
    34. Tseng, Y. C., Lee, J. R., Chang, J. C. H., Kuo, C. H., Lee, S. J., and Hwang, P. P. Regulation of lactate dehydrogenase in tilapia (Oreochromis mossambicus) gills during acclimation to salinity challenge. Zool Stud 47(4): 473-480, 2008. (Zoology; Ranking: 85/162=52 %; IF: 1.054)
    35. Tseng, Y.C., and Hwang, P. P. Some insights into energy metabolism for fish osmoregulation. Comp Biochem Physiol C Toxicol Pharmacol 148(4), 419-429, 2008. (Invited review article)
    36. Chang, J. C. H., Wu, S.M., Tseng, Y. C., Lee, Y. C., Baba, O., and Hwang, P. P. Regulation of glycogen metabolism in gills and liver of the euryhaline tilapia (Oreochromis mossambicus) during acclimation to seawater. J Exp Biol 210(19), 3494-3504, 2007.
    37. Tseng, Y. C., Huang, C. J., Chang, J. C., Teng, W. Y., Baba, O., Fann, M. J., and Hwang, P. P. Glycogen phosphorylase in glycogen-rich cells is involved in the energy supply for ion regulation in fish gill epithelia. Am J Physiol Regul Integr Comp Physiol 293(1), R482-R491, 2007.
    38. Liou, Y. M., Tseng, Y. C., and Cheng, J. C. Spectrofluorometric analysis of length-dependent conformational changes in cardiac troponin C. J Muscle Res Cell Motil 23(4), 309-315, 2002.
     

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    icob@gate.sinica.edu.tw  Copyright © ICOB 2013. All rights reserved. 最佳瀏覽網頁方式請用最新版IE11或其他瀏覽器 -- 瀏覽人數:1531887
    115台北市南港區研究院路二段128號
    Tel: 02-27899515
    Fax: 02-27858059
    icob@gate.sinica.edu.tw
    Copyright © ICOB 2013. All rights reserved. 最佳瀏覽網頁方式請用最新版IE11或其他瀏覽器 /瀏覽人數:1531887--
     瀏覽人數:1531887