當前位置:首頁>研究人員

姓名:曾庸哲

職稱:助研究員

專長:Marine ecophysiology,
Evolutionary and comparative physiology,
Systeomics

  2016/08- Assistant Research Fellow, Institute of Cellular and Organismic Biology, Academia Sinica, Taiwan
  2015/02-2016/08  Assistant Professor, Department of Life Science, National Taiwan Normal University, Taiwan
  2012/02-2015/01 Project-Appointed Assistant Professor, Department of Life Science, National Taiwan Normal University, Taiwan
  2011/07-2012/10

Distinguished Postdoctoral Fellow, Institute of Cellular and Organismic Biology , Academia Sinica, Taiwan

  2010/03-2011/08

Visiting Postdoctoral Scholar, Department of Biological Oceanography, Leibniz-Institute of Marine Sciences (IFM-GEOMAR), Germany

  2009/07-2011/06

Distinguished Postdoctoral Fellow, Institute of Cellular and Organismic Biology , Academia Sinica, Taiwan

  2009/03-2009/06

Regular Postdoctoral Fellow, Institute of Cellular and Organismic Biology , Academia Sinica, Taiwan

  2008/04-2008/10

 Postdoctoral Scholar, Department of Polar and Marine Research, Alfred Wegener Institute (AWI), Germany

  2008/04 Ph.D. Institute of Zoology, National Taiwan University, Taiwan

曾庸哲老師實驗室

  • 研究方向

    本實驗室致力於探討海洋動物個體面臨環境變遷(海洋酸化及溫度變化)之衝擊。本團隊運用次世代基因體學、分子生理學、以及細胞學等研究方法,結合臺灣四面環海的絕佳海洋生物學研究優勢,以臺灣海域的頭足類動物(軟絲、花枝、章魚)、甲殼類動物(大閘蟹、烏龜怪方蟹)、棘皮類動物(海參、海膽、陽隧足)、硬骨魚(吳郭魚、青鱂魚)為研究物種進行綜合性比較生理學研究。以求突破過往海洋生物研究的知識與傳統,準確且深入地探討海洋動物生態學表徵背後的生理學意義。

    1. 海洋動物面臨海洋酸化及暖化的衝擊

    海水表層的酸化情形往往伴隨著暖化現象的產生,可能會對於在此海域區段棲息的動物個體造成自小分子到大整體全面性的系統生理影響。而在此棲息的狹溫性動物在同時面臨高溫高碳酸刺激擾動下的生理衝擊,至今仍鮮為人知。

        本實驗室利用生物外表型模組觀測、即時生理參數檢測、次世代高速定序分析、以及基因表現相分析等研究方法,以全方位Evo-Devo-Eco(演化-發育 - 生態)的生物網絡思維研究海洋環境pH值及溫度波動對於海洋運動型生物如頭足類動物(萊氏擬烏賊及虎斑烏賊)、甲殼類動物(大閘蟹、烏龜怪方蟹) 、與硬骨魚類(鱈魚、吳郭魚、青鱂魚)胚胎及成體的生理衝擊影響。

     

    2. 動物演化生理之酸鹼與離子平衡基因體學研究

    近年來對於原始硬骨魚及四足類動物之離子和酸鹼調節等生理機制探討,已成為水生動物重要的研究議題。從開放性海域遷徙入侵至陸地淡水棲地的過程中,為了穩定地適應於低滲透壓環境,動物必須有效維持細胞內外離子梯度與進行相關的離子主動運輸。另外,當動物個體因代謝或呼吸作用,或是外界環境pH值的變動,會造成血液的酸化現象,而組織器官上皮的離子細胞為使體內pH值表持恆定,會將氫離子H+排除至血液外。藉由近十年分子生理學研究的快速進展,使得動物學家對於硬骨魚體表的「離子細胞(ionocyte)」相關生理功能有了長足且詳細的了解。

        根據最新分子生理學研究報告指出,硬骨魚胚胎與鰓上表皮離子細胞的鈉離子吸收與酸排除機制,與哺乳類腎臟近曲小管上皮細胞有著相似的同功演化機制。我們藉由整合性探討多種原始水生生物如盲鰻(Hagfish)、八目鰻(Lamprey)、鱘魚(sturgeon)、以及鯊魚(shark)離子細胞相關之碳酸酐酶(carbonic anhydrase,CA)、鈉氫交換蛋白(NHE)、V型氫離子幫浦蛋白(VHA)、與表皮鈉離子通道(epithelial Na+ channel, ENaC)的表皮離子運輸機制,以分子生理學角度重新檢視魚類上陸演化進程中體液與酸鹼恆定的演化機制。

     

    3. 水生動物因應環境變異的能量代謝及細胞抗氧化機制

    低溫緊迫或酸化波動已知會使誘發水生動物複雜的基因相變化及生化反應,並在一些重要器官強化粒線體的活性以提高代謝機制。然而粒線體提升呼吸作用的同時可能會導致生物體的傷害,包括活性氧化物質(ROS)的形成累積、粒線體內膜H+的溢漏增加、以及脂質過氧化反應的產生。其中粒線體ROS過量累積已知會造成細胞面臨氧化壓力。因此,我們透過了解水生動物HIF(低氧誘發因子)的調節、PPAR(過氧化酶體增殖劑活化受器)的路徑、UCP(解偶聯蛋白)的活化,綜合探討環境變動下動物個體內碳水化合物代謝的變化,並進行細胞抗氧化相關機制的觀察,以了解水生動物細胞能量轉移適應的生理代謝策略。

  • 姓名職稱電話Email備註
    曾庸哲助研究員
    關寶龍研究助理
    黃珮甄研究助理
    彭慧文研究助理
    劉姿延碩士班研究生
    趙仕堯碩士班研究生
    周佩璇碩士班研究生
    林孟緯大學部研習生
    陳怡安 大學部研習生
    葉蔡漢大學部研習生
    1. Weaver, R., Hill, G. E., Kuan, P. L., Tseng, Y. C. Copper exposure reduces production of red carotenoids in a marine copepod. Ecol Indic, 70, 393-400, 2016. (Environmental science; Ranking: 52/225=23 %; IF: 3.190)
    2. Hu, M. Y., Michael, K., Kreiss, C. M., Stumpp, M., Dupont, S, Tseng, Y. C.#, Lucassen, M.. Temperature modulates the effects of ocean acidification on intestinal ion transport in Atlantic cod, Gadus morhua. Front Physiol, 7, 198, 2016. (Physiology; Ranking: 20/83=24 %; IF: 4.031) (Corresponding author)
    3. Hu, M. Y., Guh, Y. J., Shao, Y. T., Kuan, P. L., Chen, G. L., Lee, J. R., Jeng, M. S., Tseng, Y. C.#. Strong ion regulatory abilities enable the crab Xenograpsus testudinatus to inhabit highly acidified marine vent systems. Front Physiol 7, 14, 2016. (Physiology; Ranking: 20/83=24 %; IF:; 4.031) (Corresponding author)
    4. Wang, Y. F., Yan, J. J., Tseng, Y. C. , Chen, R. D., Hwang, P. P. Molecular physiology of an extra-renal Cl- uptake mechanism for body fluid Cl- homeostasis. Int J Biol Sci 11(10), 1190-1203, 2015. (Biochemistry & Molecular Biology; Ranking: 63/289=22 %; IF: 3.982)
    5. Hu, M. Y., Hwang, P. P., Tseng, Y. C.# Recent advances in understanding trans-epithelial acid-base regulation and excretion mechanisms in cephalopods. Tissue Barriers 3(4), e1064196. (Invited review article)
    6. Stumpp, M., Hu, M. Y., Tseng, Y. C., Guh, Y. J., Chen, Y. C., Su, Y. H., Yu, J. K., Hwang, P. P. Evolution of extreme pH in bilaterian digestive systems inferred from gastric alkalization mechanisms in basal deuterostomes. Sci Rep 5, 10421, 2015. (Multidisciplinary science; Ranking: 5/56=9 %; IF: 5.228)
    7. Furukawa, F., Tseng, Y. C., Liu, S. T., Chou, Y. L., Lin, C. C., Sung, P. H., Uchida, K., Lin, L. Y., Hwang, P. P. Induction of phosphoenolpyruvate carboxykinase (PEPCK) during acute acidosis and its role in acid secretion by V-ATPase-expressing ionocytes. Int J Biol Sci 16(2), 712-725, 2015. (Biochemistry & Molecular Biology; Ranking: 63/289=22 %; IF: 3.982)
    8. Shao, Y. T., Tseng, Y. C.#, Chang, C. H., Yan, H. Y, Hwang, P. P., Borg, B. GnRH mRNA levels in male three-spined sticklebacks, Gasterosteus aculeatus, under different reproductive conditions. Comp Biochem Physiol A Mol Integr Physiol 180, 6-17, 2015. (Zoology; Ranking: 31/153=20 %; IF: 2.039) (Corresponding author)
    9. Hu, M. Y., Guh, Y. J., Stumpp, M., Lee, J. R., Chen, R. D., Sung, P. H., Chen, Y. C., Hwang, P. P. and Tseng, Y. C.#. Branchial NH4+-dependent acid-base transport mechanisms and energy metabolism of squid (Sepioteuthis lessoniana) affected by seawater acidification. Front Zool 11, 55, 2014. (Zoology; Ranking: 9/153=6 %; IF: 3.042) (Corresponding author)
    10. Tseng, Y. C., Liu, S. T., Hu, M. Y., Chen, R. D., Lee, J. R., and Hwang, P. P. Brain functioning under acute hypothermic stress supported by dynamic monocarboxylate utilization and transport in ectothermic fish. Front Zool 11, 53, 2014. (Zoology; Ranking: 9/153=6 %; IF: 3.042) (First author)
    11. Hsu, H. H., Lin, L. Y., Tseng, Y. C., Horng, J. L., and Hwang, P. P. A new model for fish ion regulation: Identification of ionocytes in freshwater- and seawater-acclimated medaka (Oryzias latipes). Cell Tissue Res 357(1), 225-243, 2014. (Cell Biology; Ranking: 78/184=42%; IF: 2.948)
    12. Guh, Y. J., Tseng, Y. C., Yang, C. Y., and Hwang, P. P. Endothelin-1 regulates H+-ATPase-dependent transepithelial H+ secretion in zebrafish. Endocrinology 155(5), 1728-1737, 2014. (Endocrinology & Metabolism; Ranking: 28/128=22%; IF: 4.159)
    13. Lee, L. C., Wang, Y. T., Wu., Y. R., Soong, B. W., Tseng, Y. C., Chen, C. M., Lee, G. J. Downregulation of proteins involved in the endoplasmic reticulum stress response and Nrf2-ARE signaling in lymphoblastoid cells of spinocerebellar ataxia type 17. J Neural Transm 121(6), 301-610, 2014. (Clinical neurology; Ranking: 90/192=47%; IF: 2.587)
    14. Chen, C. M., Wu, C. H., Hsieh, C. H., Lin, C. H., Chen, I. C., Chen, Y. C., Lee, L. C., Lee, C. M., Tseng, Y. C., Lee, G. J., and Wu, Y. R. HTRA2 variations in Taiwanese Parkinson’s disease. J Neural Transm 121(5), 491-198, 2014. (Clinical neurology; Ranking: 90/192=47%; IF: 2.587)
    15. Hu, M. Y., Lee, J. R., Lin, L. Y., Shih, T. H., Stumpp,M., Lee, M. F., Hwang, P. P. and Tseng, Y. C.#. Development in a naturally acidified environment: Na+/H+-exchanger 3-based proton secretion leads to CO2 tolerance in cephalopod embryos. Front Zool 10(1), 51, 2013. (Zoology; Ranking: 9/153=6 %; IF: 3.042) (Corresponding author)
    16. Tseng, Y. C., Hu, M. Y., Stumpp, M., Lin, L. Y., Melzner, F., and Hwang, P. P. CO2-driven seawater acidification differentially affects development and molecular plasticity along life history of fish (Oryzias latipes). Comp Biochem Physiol A Mol Integr Physiol 165(2), 119-130, 2013. (Zoology; Ranking: 31/153=20 %; IF: 2.039) (First author)
    17. Tseng, Y. C., Kao, Z. J., Liu, S. T., Chen, R. D., and Hwang, P. P. Spatial expression and functional flexibility of monocarboxylate transporter isoforms in the zebrafish brain. Comp Biochem Physiol A Mol Integr Physiol 165(2), 106-118, 2013. (Zoology; Ranking: 31/153=20 %; IF: 2.039) (First author)
    18. Shao, Y. T., Tseng, Y. C., Trombley, S., Hwang, P. P., Schmitz, M., and Borg, B. Schistocephalus solidus infections increase gonadotropins and gonadotropin releasing hormone (GnRH3) mRNA levels in the three-spined stickleback, Gasterosteus aculeatus. Parasitol Int 61(3), 470-474, 2012. (Parasitology; Ranking: 16/36=44%; IF: 1.860)
    19. Hu, M. Y.*, Tseng, Y. C.*, Lin, L. Y., Chen, P. Y., Charmantier-Daures, M., Hwang, P. P., and Melzner, F. New insights into ion regulation of cephalopod molluscs: a role of epidermal ionocytes in acid-base regulation during embryogenesis. Am J Physiol Regul Integr Comp Physiol 301(6), R1700-R1709, 2011. (* Equal Contribution) (Physiology; Ranking: 25/83=30 %; IF: 3.168)
    20. Tseng, Y. C., Chen, R. D., Lucassen, M., Schmidt, M. M., Abele, D., and Hwang, P. P. Exploring uncoupling proteins and anti-oxidative stress mechanisms under acute cold exposure in brains of fish. PLoS ONE 6(3), e18180, 2011. (Multidisciplinary Sciences; Ranking: 8/56=14 %; IF: 3.057) (First author)
    21. Hu, M. Y., Tseng, Y. C., Stumpp, M., Gutowska, M. A., Kiko, R., Lucassen, M., and Melzner, F. Elevated seawater pCO2 differentially affects branchial acid-base transporters over the course of development in the cephalopod Sepia officinalis. Am J Physiol Regul Integr Comp Physiol 300(5), R1100-R1114, 2011. (Physiology; Ranking: 25/83=30 %; IF: 3.168)
    22. Tseng, Y. C., Lee, J. R., Lee, S. J., and Hwang, P. P. Functional analysis of glucose transporter 1a, -6, and -13.1 expressed in epithelial cells. Am J Physiol Regul Integr Comp Physiol 300(2), R321-R329, 2011. (Physiology; Ranking: 25/83=30 %; IF: 3.168) (First author)
    23. Lafont, A. G., Wang, Y. F., Chen, G. D., Liao, B. K., Tseng, Y. C., Hwang, C. J., and Hwang, P. P. Involvement of calcitonin and its specific receptor in the control of calcium regulating-genes and calcium homeostasis in zebrafish (Danio rerio). J Bone Miner Res 26(5), 1072-1083, 2011. (Endocrinology & Metabolism; Ranking: 12/128=9 %; IF: 5.622)
    24. Cruz, S., Tseng, Y. C., Kaiya, H., and Hwang, P. P. Ghrelin affects carbohydrate-glycogen metabolism via insulin inhibition and glucagon stimulation in the zebrafish (Danio rerio) brain. Comp Biochem Physiol A Mol Integr Physiol 156(2), 190-200, 2010. (Zoology; Ranking: 31/153=20 %; IF: 2.039)
    25. Tseng, Y. C., Chen, R. D., Lee, J. R., Liu, S. T., Lee, S. J., and Hwang, P. P. Specific expression and regulation of glucose transporters in zebrafish ionocytes. Am J Physiol Regul Integr Comp Physiol 297(2), R275-R290, 2009. (a congratulation letter from the editor) (Physiology; Ranking: 25/83=30 %; IF: 3.168) (First author)
    26. Wang, Y. F., Tseng, Y. C., Yan, J. J., Hiroi, J., and Hwang, P. P. Role of SLC12A10.2, a Na-Cl cotransporter-like protein, in a Cl uptake mechanism in zebrafish (Danio rerio). Am J Physiol Regul Integr Comp Physiol 296(5), R1650-R1660, 2009. (a congratulation letter from the editor) (Physiology; Ranking: 25/83=30 %; IF: 3.168)
    27. Tseng, D. Y., Chou, M. Y., Tseng, Y. C., Hsiao, C. D., Huang, C. J., Kaneko, T., and Hwang, P. P. Effects of Stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo. Am J Physiol Regul Integr Comp Physiol 296(3), R549-R557, 2009. (Physiology; Ranking: 25/83=30 %; IF: 3.168)
    28. Tseng, Y. C., Lee, J. R., Chang, J. C. H., Kuo, C. H., Lee, S. J., and Hwang, P. P. Regulation of lactate dehydrogenase in tilapia (Oreochromis mossambicus) gills during acclimation to salinity challenge. Zool Stud 47(4): 473-480, 2008. (Zoology; Ranking: 112/153=73 %; IF: 0.885) (First author)
    29. Tseng, Y.C., and Hwang, P. P. Some insights into energy metabolism for fish osmoregulation. Comp Biochem Physiol C Toxicol Pharmacol 148(4), 419-429, 2008. (Invited review article) (Zoology; Ranking: 24/153=16 %; IF: 2.546) (First author)
    30. Chang, J. C. H., Wu, S.M., Tseng, Y. C., Lee, Y. C., Baba, O., and Hwang, P. P. Regulation of glycogen metabolism in gills and liver of the euryhaline tilapia (Oreochromis mossambicus) during acclimation to seawater. J Exp Biol 210(19), 3494-3504, 2007. (Biology; Ranking: 18/85=21 %; IF: 2.914)
    31. Tseng, Y. C., Huang, C. J., Chang, J. C., Teng, W. Y., Baba, O., Fann, M. J., and Hwang, P. P. Glycogen phosphorylase in glycogen-rich cells is involved in the energy supply for ion regulation in fish gill epithelia. Am J Physiol Regul Integr Comp Physiol 293(1), R482-R491, 2007. (Physiology; Ranking: 25/83=30 %; IF: 3.168) (First author)
    32. Liou, Y. M., Tseng, Y. C., and Cheng, J. C. Spectrofluorometric analysis of length-dependent conformational changes in cardiac troponin C. J Muscle Res Cell Motil 23(4), 309-315, 2002. (Cell biology; Ranking: 138/187=74 %; IF: 2.071)

  • 115台北市南港區研究院路二段128號  Tel: 02-27899515   Fax: 02-27858059  *個人隱私權聲明*
    icob@gate.sinica.edu.tw  Copyright © ICOB 2013. All rights reserved. 最佳瀏覽網頁方式請用最新版IE11或其他瀏覽器 -- 瀏覽人數:1029514
    115台北市南港區研究院路二段128號
    Tel: 02-27899515
    Fax: 02-27858059
    icob@gate.sinica.edu.tw
    Copyright © ICOB 2013. All rights reserved. 最佳瀏覽網頁方式請用最新版IE11或其他瀏覽器 /瀏覽人數:1029514--
     瀏覽人數:1029514