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姓名:黃聲蘋

職稱:副研究員兼副所長

電話:

專長:Molecular Biology,
Gene Regulation,
Developmental Biology

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研究人員 / 簡介
  August 2010-present Associate Research Fellow  Tenured  of  ICOB, Academia Sinica, Taiwan
  2005-, Associate Research Fellow of ICOB, Academia Sinica, Taiwan
  1993-2005, Associate Research Fellow of IZ, Academia Sinica, Taiwan
  1990-1993 Postdoctoral Research Associate, Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, USA
  1989, Ph.D. Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, USA

黃聲蘋 老師 實驗室

  • 主要的研究方向

    以斑馬魚為模式生物來探討胚胎發育過程中器官形態形成所參與的分子調控機制,目前的研究著重於有關於消化道、表皮、咽弓及心臟等器官形成的基因調控機制。我們亦發展基因轉殖斑馬魚及CRISPR-Cas基因編輯所產生的變種魚來幫助上述器官形態形成時基因調控機制的研究。在消化道器官形態形成方面:我們的研究顯示斑馬魚caudal-related homeobox Cdx1b 會調節Nodal 訊息傳遞路徑成員如 foxa2gata5 的表現來控制早期內胚層細胞的形成。Cdx1b 在胚胎後期不同腸道細胞如杯狀細胞、腸道內分泌細胞及 enterocyte 的分化及腸道細胞增生擔任重要的功能。斑馬魚轉錄因子 Krüppel-like factor 4a (Klf4a)的表現 則會受Notch signaling調控來影響腸道杯狀細胞的分化,同時 Klf4a會抑制腸道細胞的增生。斑馬魚 agr2 表現在大多數的器官如表皮、嗅覺、耳、咽喉、食道、pneumatic duct、魚膘及腸道等具有黏液分泌的細胞中。Morpholino 弱化的實驗顯示斑馬魚 Agr2 是腸道杯狀細胞末端分化的必要蛋白質。當分析 agr2表現的調控機制,除了找到Foxa2及Hif1ab轉錄因子會結合在 agr2基因上游的特定DNA序列,來調控 agr2 表現在腸道杯狀細胞內及促進杯狀細胞的末端分化,我們也找到調控agr2 表現在內耳的DNA序列,並發現 Sox10會結合在此序列來調控agr2表現在內耳的半規管及sensory patches中的支持細胞中。在心臟器官的形態形成方面:我們利用 BMP4 心臟專一的啟動子及其上游調控序列,製備一Tg(BMP4:EGFP)as10 基因轉殖魚,其胚胎具有表現綠色螢光蛋白在心室及心房的心肌內。利用此基因轉殖魚進行ENU化學突變所製備的一突變魚 S1pr2as10 ,其心臟前驅細胞移動有缺陷造成兩個心臟的形成。我們發現當培養此突變魚胚胎於低溫下會拯救其兩個心臟的缺陷,並發現低溫會誘導過氧物的產生,續而調控細胞間質基因如fibronectin 1 tenascin-c tenascin-w 的表現來促進心臟前驅細胞的正常移動。在表皮器官的形態形成方面:我們有興趣探討表皮黏液細胞的分化機制。

     

     

    Fig. No EGFP expression can be detected in transgenic embryos expressing mutated binding motifs of HRE and FHRE.

     

    Fig. Foxa1 binds to FHRE motif to regulate agr2 expression in the pharynx while both Hif1ab and Foxa2 bind to HRE or FHRE motifs to regulate agr2 expression in the intestinal goblet cells and the maturation of these cells.

     

     

     


     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

  • 姓名職稱電話Email備註
    黃聲蘋副研究員
    陳宜群博士後
    呂玉芬研究助理
    吳淳繡博士生
    謝芳琪博士生
    劉昱秀博士生
    曾華斌博士後
    陳美貞魚房助理
    蕭瑋銓碩士生
  • Selected Publications

    1. Shentu, H., Wen, H-J., Her, G-M., Huang, C-J., Wu, J-L., and Hwang, S-P.L.* (2003). Proximal upstream region of zebrafish bone morphogenetic protein 4 promoter directs heart expression of green fluorescent protein. Genesis 37: 103-112.
    2. Wang, W. D., Huang, C. J., Lu, Y. F., Hsin, J. P. Prabhakar, V. R. Cheng, C. F., and Hwang, S-P.L.* (2006). Heart-targeted overexpression of Nip3a in zebrafish embryos causes abnormal heart development and cardiac dysfunction. Biochem. Biophys. Res. Commun. 347: 979-987.
    3. Shih, L. J., Lu, Y. F., Chen, Y. H., Lin, C. C., Chen, J. A., and Hwang, S-P.L.* (2007). Characterization of the Agr2 gene, a homologue of X. laevis anterior gradient 2, from the zebrafish, Danio rerio. Gene Expression Patterns 7:452-460.
    4. Cheng, P-Y., Lin, C-C., Wu, C-S., Lu, Y-F., Lin, C Y., Chung, C-C., Chu, C-Y., Huang, C-J., Tsai, C-Y., Korzh, S., Wu, J-L., and Hwang, S-P.L.* (2008). Zebrafish cdx1b regulates expression of downstream factors of Nodal signaling during early endoderm formation. Development 135: 941-952.
    5. Chen, Y-H., Lu, Y-F., Ko, T-Y., Tsai, M-Y., Lin, C-Y., Lin, C-C., and Hwang, S-P. L.* (2009). Zebrafish cdx1b regulates differentiation of various intestinal cell lineages. Dev. Dyn. 238:1021-1032.
    6. Li, I-C., Chan, C-T., Lu, YF., Wu, Y-T., Chen, YC., Li, G-B., Lin, C-Y., Hwang, S-P.L.* (2011). Zebrafish Krüppel-like factor 4a represses intestinal cell proliferation and promotes differentiation of intestinal cell lineages. PLoS one 6(6): e20974. doi:10.1371/journal.pone.0020974
    7. Wu, Y-T., Lin, C-Y., Tsai, M-Y., Chen, Y-H., Lu, Y-F., Huang, C-J., Cheng, C-M., Hwang, S-P.L.* (2011). β-Lapachone induces heart morphogenetic and functional defects by promoting the death of erythrocytes and the endocardium in zebrafish embryos. J. Biom. Sci. 18:70.
    8. Chen, Y-C., Lu, Y-F., Li, I-C., Hwang, S-P.L.* (2012). Zebrafish Agr2 is required for terminal differentiation of intestinal goblet cells. PLoS ONE 7(4): e34408.
    9. Lin, C-Y., Huang, C-C., Wang, W-D., Hsiao, C-D., Cheng, C-F., Wu, Y-T., Lu, Y-F., Hwang, S-P.L.* (2013). Low temperature mitigates cardia bifida in zebrafish embryos. PLoS ONE 8(7): e69788.
    10. Tang, C-H., Lai, Y-R., Li, C-H., Chen, Y-C, Lu, Y-U., Chen, H-Y., Lien, H-W., Yang, C-H., Huang, C-J., Wang, C-Y., Kao, C-F., Hwang, S-P. L.,* (2014). Expression of zebrafish anterior gradient 2 in the semicircular canals and supporting cells of otic vesicle sensory patches is regulated by Sox10. Biochimica et Biophysica Acta 1839: 425-437.
    11. Tsai, M-Y., Lu, Y-F., Liu, Y-H., Lien, H-W., Huang, C-J., Wu, J-L., Hwang, S-P. L.,* (2014). Modulation of p53 and met expression by Krüppel-like factor 8 regulates zebrafish cerebellar development. Developmental Neurobiology 75: 908–926.
    12. Lai, Y-R., Lu, Y-F., Lien, H-W. Huang, C-J. Hwang, S-P. L.* (2016). Foxa2 and Hif1ab regulate maturation of intestinal goblet cells by modulating agr2 expression in zebrafish embryos. Biochem. J. 473: 2205-2218.
    13. You, M-S*., Jiang, Y-J., Yuh, C-H., Wang, C-M., Tang, C-H., Chuang, Y-J., Lin, B-H., Wu, J-L., Hwang, S-P. L.* (2016). A sketch of the Taiwan Zebrafish Core Facility. Zebrafish Suppl 1: S24-29.
       

     


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    115台北市南港區研究院路二段128號
    Tel: 02-27899515
    Fax: 02-27858059
    icob@gate.sinica.edu.tw
    Copyright © ICOB 2013. All rights reserved. 最佳瀏覽網頁方式請用最新版IE11或其他瀏覽器 /瀏覽人數:897558--
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