Biological Electron Microscopy
 
Introduction
 

      The Biological Electron Microscopy (EM) Core Facility is a research support facility administered and supported by ICOB and the AS Core Facility and Innovative Instrument Project. Our mission is to provide researchers with technical consultation and operational training on EM sample preparation. We are open to all faculty, staff and students on the entire Academia Sinica campus, and also to off-campus investigators and users. The facility is equipped with one scanning and one transmission electron microscope in addition to all of the required ancillary equipment to carry out sample processing for imaging from the tissue to the molecular level.
Sample preparations we support (cells, tissues, and synthetic soft materials) include: negative stain, heavy metal shadowing, conventional resin embedding/sectioning, high pressure freeze, and freeze-substitution embedding/sectioning.

envir
Environmental Scanning Electron Microscope (FEI Quanta 200)
 Transmission Electron Microscope (FEI Tecnai G2 F20 S-TWIN)  
 

 

 
Equipment Manager:Po-Yen Lin     Tel-9526   
 

Items

Equipment

Brand/Model

Applications

Status
1

Transmission Electron Microscope

 

FEI Tecnai G2 F20 S-TWIN

The Tecnai G2 F20 S-TWIN is a 200 kV/FEG Transmission Electron Microscope with a Gatan UltraScan 1000 (2k x2k) CCD camera. It is used to collect high-resolution images and tilt image series for electron tomography. The instrument is also equipped with a STEM device coupled to an EDX spectrometer for elemental analysis.

OK
2

Environmental Scanning Electron Microscope

 

FEI Quanta 200

The Quanta 200 SEM is a versatile high performance, low-vacuum scanning electron microscope with a tungsten electron source. The Quanta has three imaging modes (high vacuum, low vacuum and ESEM), which can accommodate the widest range of samples of any SEM system.

OK
3 High Pressure Freezer Leica EM HPM101

This High Pressure Freezer provides a cryo-fixation platform to better preserve antigens for immuno-EM. Unlike chemical fixation, which results in sample shrinkage and extraction, high pressure freezing maintains the natural structural integrity of cells and tissue samples.

OK
4 Freezing Substitution Leica EM AFS

After high pressure freezing, samples are moved to the Leica EM AFS2 to perform freeze substitution, allowing low-temperature embedding and polymerization of resins.

OK
5

Ultramicrotome

Leica EM UC7

This instrument can be used to generate ultra-thin sections of resin sample blocks.

OK
6

Glow discharge

EMITECH K100X

The K100X is a simple, free-standing glow discharge system, typically used for hydrophobic to hydrophilic conversion of carbon-coated TEM grids

OK
7   Denton Vacuum This sputter coater provides thin film deposition of Pt/Pd, W or carbon on a variety of substrates OK
 
Policies Manager:Po-Yen Lin     Tel-9526   
 
  • Training is required for each instrument before booking
  • EM users are categorized into two levels: Regular and Experienced.
  Regular users can only operate software to take EM images; EM staff will assist in changing the samples. Experienced users may change EM grids and operate the EM during off-hours. An assessment is required for upgrade of user status.
  • For TEM and SEM, the maximum booking frequency is 2 days per week for each lab. However, users may book more than 2 days per week to fill empty time slots.
  • Please cancel reservations 48 hours in advance of the scheduled booking. Otherwise, charges will apply based on the time reserved.
  • All users must clearly sign the work log with user info.
  • Reservations for the TEM and SEM are made through the IIS reservation system. For off-hour usage, please contact the EM staff.

 

 
Usage protocol Manager:Po-Yen Lin     Tel-9526 
 
How to apply
EM sample preparation:
Discuss your experiment and sample conditions --> fill out the application form à process samples --> inform the user when EM samples are ready.

EM imaging:
If your EM samples are prepared by yourself, you may book the EM through the IIS reservation system. The EM staff will assist you in EM imaging.  

Training:
We provide one-on-one training for EM sample preparation, including chemical fixation, resin embedding, ultrathin section and staining.

Pricelist

We appreciate if users acknowledge the Biological Electron Microscopy Core Facility when publishing data from our EM services is. An example acknowledgement follows:

Acknowledgment
We thank the Academia Sinica Biological Electron Microscopy Core Facility for EM technical support. The core facility is funded by the Academia Sinica Core Facility and Innovative Instrument Project (AS-CFII-108-119).

 

SOP
HPF&FS-RT-TEM protocol(Morphology of Yeast) HPF&FS-TEM protocol (IEM)
ImmunoGold Labeling for Sections ImmunoNegative Staining for Particles
Negative Staining for Particles Plunge&FS-TEM protocol(IEM of tissues & bacteria)
Standard Operating Procedures-LT Standard Operating Procedures-RT

 

 

 

 

 

 

128 Sec.2, Academia Rd, Nankang, Taipei 115 Taiwan, R.O.C Tel: 02-27899515 Fax: 02-27858059

icob@gate.sinica.edu.tw Copyright © ICOB 2013. All rights reserved.