Light Microscopy
Introduction
 

    The Light Microscopy Core Facility is located in room 401 at the Institute of Cellular and Organismic Biology. We provide experimental consultation, training in imaging acquisition platforms, and image analysis software packages. Access is typically reserved for ICOB-affiliated researchers. Please contact the Core staff for details.


Services include:

  • User training for acquisition platforms.
  • Access to wide-field and fluorescence light microscopy.
  • Access to live imaging microscopy.
  • User training for image analysis.
  • Customized macro editing.

Equipment available:

  • Inverted fluorescence microscope with live-cell imaging and motorized stage.
  • Upright fluorescence microscopes.
  • Stereo fluorescence microscope.
  • Slide scanner.
  • Workstations for image processing

 
 
Equipment Manager:Peggy Hsu   Tel-1531  
 

Leica Laser MicroDissection 7

Objective list

  • UVI 5x/0.12 Microdissection
  • HC PL FLUOTAR 10x/ 0.32
  • HC PL FLUOTAR L 20x/ 0.40 corr
  • HC PL FLUOTAR L 40x/0.6 corr
  • HC PL FLUOTAR L 63x/0.7 corr XT

Applications

  • Laser microdissection

Control software

  • LMD
 

Zeiss Axio Observer

Objective list

  • Plan 10x/0.25
  • LD Plan-Neofluar 20x/0.40 corr
  • LD Plan-Neofluar 40x/0.60 corr
  • Plan-Apochromat 63x/1.40 Oil
  • Plan-Apochromat 100x/1.40 Oil

Applications

  • Cell morphology acquisition
  • Tile scan
  • Plate scan
  • Live cell imaging

Control software

  • Zen Blue
 

Leica DM6000B

Objective list

  • PL Fluotar 2.5x/0.07
  • HC PLAPO 10x/ 0.4
  • HC PLAPO 20x/ 0.70
  • N Plan L 40x/0.55 Korr
  • HCX Plan APO 100x/1.35 oil

Applications

  • Cell morphology
  • Histological imaging

Control software

  • MetaMorph
 

Zeiss Axioplan 2 Imaging

Objective list

  • Plan-Neofluar 5x/ 0.15
  • Plan-Apochromat 10x/0.45
  • Plan-Apochromat 20x/0.75
  • Plan-Apochromat 40 /0.95
  • Plan-Apochromat 63x/1.40 Oil
  • Plan-Apochromat 100x/1.40 Oil

Applications

  • Cell morphology
  • Histological imaging

Control software

  • AxioVision

 

Zeiss SV11 Apo

Objective list

  • HR 10x/0.45  
  • PL APO 20x/0.42

Applications

  • Dissection or observation under transmitted light
  • Dissection or observation under reflected light
  • Dissection or observation under fluorescence

Control software

  • AxioVision

 

TissueGnostics Slide Scanner

Objective list

  • EC Plan Neofluar 2.5x/0.075
  • EC Plan Neofluar 10x/0.3
  • EC Plan Neofluar 20x/0.5
  • EC Plan Neofluar 40x/1.3 oil

Applications

  • Cell morphology
  • Histological imaging

Control software

  • TissueFAXS
 

Amira workstation

 

Imaris workstation

 

MetaMorph workstation

 

LAS-AF workstation

 

Zen workstation

 
Policies Manager:Peggy Hsu    Tel-1531  
 
  • Access is typically reserved for ICOB-affiliated personnel.
  • Individuals must have received training and certification as independent users before using equipment unassisted or outside of regular business hours.
  • The Imaging Core Facility is open 24 hours per day, 7 days per week with the access control.
  • Only certified users may apply to access the confocal or light microscope room.
  • The reservation system allows scheduling up to 13 days in advance.
  • In order to allow us to track and bill for utilization, all users must make reservation and sign in online prior to each period of use. 
  • If you are more than 20 minutes late during high-use periods, your time slot may be assigned to another user.
  • If you overbook time (i.e., book three hours and only use one hour), please sign out online. The booking system will send a notice to other users and release the time slot for booking.
  • Please cancel your reservation if you will not use facility. Otherwise, your booking account will be suspended for one month.
  • All users must clearly sign the work log with user name, PI name and the usage time.
  • If you have any questions or encounter trouble with the equipment, please contact the Core staff immediately. Replacing any accessories and changing the settings in the control software is not allowed.
  • All users are obligated to keep the facilities clean, including the environment and all objectives (especially immersed objectives).
  • No drinking and eating are allowed in the confocal and light microscope rooms.
  • Experimental consumables and packages of food are not allowed to be left in the confocal and light microscope room, including the trash can.
  • All users must use their own badges to enter confocal and microscope rooms.
  • Anyone who violates the above policies will have their access to the room revoked and their PI will be informed of the violation

Pricing

Use of the fluorescence light source          $ 50 / hr
Extended usage of the fluorescence light source  $ 12.5 / 15 min

Billing

  • All users must clearly sign the work log with their user name, PI name and the usage time for billing purposes.
  • Costs will be charged hourly and billed monthly.
  • Every 15 minutes of extended use will be charged $12.5.
  • No charge will be levied for less than 15 minutes.
Acknowledgement of the Core Facility

All users of the Imaging Core Facility should acknowledge the facility in publications and presentations that include data generated in the facility. Please also acknowledge the macro editor who writes the customized macro or script. We also appreciate receiving an electronic version of publications that include data gathered in our facility.

Acknowledgment template

We thank the Imaging Core Facility at the Institute of Cellular and Organismic Biology, Academia Sinica, for the technical support in image acquisition and analysis.

 
Usage protocol Manager:Peggy Hsu    Tel-1531  

Service object
   ICOB members

Apply for access

  1. Contact the Core staff to arrange a training course.
  2. Contact the Core staff to become certified as an independent user.
  3. Fill out the application form. (Download)
  4. Submit a hard copy of the application to the computer room.

How to use

  1. Make a reservation online. The reservation system allows scheduling 13 days in advance. (Link)
  2. Log in online before use.(Link)
  3. Fill out the work log on the desk.
  4. Log out online after use. (Link)

Hours

    24 hours per day, 7 days per week

 

 
Contact us  
 

Peggy Hsu, PhD
Phone: 886-2-27871531
Email: peggyschsu@gate.sinica.edu.tw

 

 

 

 

128 Sec.2, Academia Rd, Nankang, Taipei 115 Taiwan, R.O.C Tel: 02-27899515 Fax: 02-27858059

icob@gate.sinica.edu.tw Copyright © ICOB 2013. All rights reserved.