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Cheng-Fu Kao|Institute of Cellular and Organismic Biology, Academia Sinica

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  • Cheng-Fu Kao
    Research Fellow & Deputy Director
    • SpecialtyChromatin Structure & Dynamics, DNA Replication and Repair
    • E-maickao@gate.sinica.edu.tw
    • Tel02-2787-1515
    • Website Cheng-Fu Kao's Lab
    • LabR234/ICOB
Professional ExperienceOpenClose
Research fellow, Institute of Cellular and Organismic Biology, Academia Sinica
Associate research fellow, Institute of Cellular and Organismic Biology, Academia Sinica
2006- 2015
Assistant research fellow, Institute of Cellular and Organismic Biology, Academia Sinica
2005- 2006
Research Faculty, Dept. of Molecular and Microbiology, University of New Mexico
2002- 2005
Postdoctoral Research Fellow, Dept. of Molecular and Microbiology, University of New Mexico
University of Edinburgh Ph. D, Biochemistry
Research InterestOpenClose

• Chromatin structure and function

• Metabolism on chromatin dynamics and epigenetics

• Chromatin regulatory mechanisms in development and disease

The research in my laboratory centers on the molecular mechanisms that determine Chromatin structure and Cellular functions. The density and positioning of nucleosomes sterically determine the ability of cellular machinery to access the genome. Consequently, chromatin structure has a crucial role in diverse processes including activating or repressing transcription to control functions such as cell fate, the cell cycle, and DNA damage repair. A critical component of chromatin-mediated regulation uses histone post-translational modifications (PTMs), in which histones integrate cellular signals to compose chromatin-dependent functions. Given the regulatory role of chromatin for all DNA-templated processes, it is not surprising that many developmental syndromes are caused by mutations in the protein machinery that ‘writes’, ‘reads’ and ‘erases’ these histone marks and their regulations are frequently altered in cancer, and in many cases these mutations are oncogenic drivers or contributors to tumor progression.

Selected PublicationOpenClose
  1. Lin CY, Chang YM, Tseng HY, Shih YL, Yeh HH, Chen PL, Chen CC, Yan YT*, and Kao CF*. Chromatin state transition underlies the temporal changes in gene expression during cardiomyocyte maturation. bioRxiv 2021.12.28.474318.
  2. Huang JH, Liao YR, Lin TC, Tsai CH, Lai WY, Chou YK, Leu JY, Tsai HK*, Kao CF*. iTARGEX analysis of yeast deletome reveals novel regulators of transcriptional buffering in S phase and protein turnover. Nucleic Acids Res. 2021 Jul 21;49(13):7318-7329. doi: 10.1093/nar/gkab555.
  3. Hsu CL, Chong SY, Lin CY, Kao CF*. Histone dynamics during DNA replication stress. J Biomed Sci. 2021 Jun 19;28(1):48. doi: 10.1186/s12929-021-00743-5.
  4. Chang CY, Hung JH, Huang LW, Li J, Fung KS, Kao CF, Chen L. Epigenetic Regulation of WNT3A Enhancer during Regeneration of Injured Cortical Neurons. Int J Mol Sci. 2020 Mar 10;21(5). pii: E1891. doi: 10.3390/ijms21051891. 
  5. Chang CY, Liang MZ, Wu CC, Huang PY, Chen HI, Yet SF, Tsai JW, Kao CF*, Chen L*. WNT3A Promotes Neuronal Regeneration upon Traumatic Brain Injury. Int J Mol Sci. 2020 Feb 21;21(4). pii: E1463. doi: 10.3390/ijms21041463. (
  6. Chong SY, Cutler S, Lin JJ, Tsai CH, Tsai HK, Biggins S, Tsukiyama T, Lo YC and Kao CF*. H3K4 methylation at active genes mitigates transcription-replication conflicts during replication stress. Nat Commun. 2020 Feb 10;11(1):809. doi: 10.1038/s41467-020-14595-4. 
  7. You ST, Jhou YT, Kao CF, Leu JY. Experimental evolution reveals a general role for the methyltransferase Hmt1 in noise buffering. PLoS Biol. 2019 Oct 15;17(10):e3000433. 
  8. Wu MY, Lin CY, Tseng HY, Hsu FM, Chen PY and Kao CF*. H2B ubiquitylation and the Asf1 histone chaperone mediate the formation and maintenance of heterochromatin architecture. Nucleic Acids Res. 2017 May 17. doi: 10.1093/nar/gkx422. (*correspondent author) 
  9. Hung SH, Wong RP, Ulrich HD* and Kao CF*. Bre1-mediated mono-ubiquitylation of H2B contributes to the bypass of DNA damage during and after DNA replication Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2205-E2214. (*correspondent authors) 
  10. Chen KW, Chang YJ, Yeh CM, Lian YL, Chan MW, Kao CF* and Chen L. SH2B1 modulates chromatin state and MyoD occupancy to enhance expressions of myogenic genes. Biochim Biophys Acta. 2017 Feb;1860(2):270-281.