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研究人員｜中央研究院 細胞與個體生物學研究所

研究人員
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側邊選單開關 研究人員
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蘇怡璇老師實驗室

  • 蘇怡璇Yi-Hsien Su
    研究員Research Fellow
    • 專長：Developmental Biology, Gene Regulatory Networks, Systems Biology
    • 信箱：yhsu@gate.sinica.edu.tw
    • 電話：02-2789-9511
    • 位置：R410/ICOB
友善列印
實驗室簡介展開收合
SeaUrchinLateDevelopment.jpg
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PNAS_Cover_Su.jpg

動物的發育是由調節性基因形成的複雜網路所調控，基因調節網路描述了訊息傳遞分子如何活化轉錄因子以及轉錄因子與DNA的結合調控了調節性基因在發育過程中適時適地的表現。這些在某特定發育時期的某些細胞所表現的調節性基因進而掌控了細胞的分化、器官的形成以及動物身體體制的發育。

本實驗室的研究主要著重在瞭解訊息分子如何調控轉錄因子以組成發育基因調控網路，我們特別感興趣的問題在於研究調控動物身體體制與體軸的分子機制，我們使用海膽胚胎來研究相關的課題。海膽屬於棘皮動物，是生活在海洋中的無脊椎動物。海膽被用來做為發育生物學的研究材料已有超過百年的歷史，其大量的精卵、簡易的體外受精、透明且同步發育的胚胎、卵裂及發育的方式（與脊椎動物同為後口動物，第一個產生的胚口會形成肛門），都讓它成為傳統上研究以及課堂上展示給學生觀察胚胎發育極佳的動物模式。

近年來因為顯微注射技術的發展以及基因體序列的解析，海膽胚胎亦是現代研究發育基因調控網路重要的模式生物之一。在海膽的發育過程中，其身體體制會發生數次的轉換，它們會由輻射對稱的胚胎發育成兩側對稱的浮游性幼生，之後才會發展出五幅對稱的成體構造。在這個兩側對稱轉換成五幅對稱的發育過程中，因為左右不對稱的調控機制使五幅對稱的雛體只在浮游性幼生的左邊形成，之後幼生的組織逐漸退化，最後進一步變態成五幅對稱的幼體。

本實驗室過去的研究發現BMP訊息分子會調控胚胎的背腹軸及左右軸，同時也發現數個訊息分子與體軸的建立有關。我們近年來也開始研究台灣半索動物玉柱蟲的發育機制。因為半索動物和棘皮動物為最接近的兩個動物門，與脊索動物同為後口動物的三大動物門，我們希望藉由比較兩者的發育分子機制，來探討後口動物身體體制的演化。

 

【海膽】細胞和動物模式小學堂_2021中研院細生所線上院區開放

榮譽獎項展開收合

2020 科技部傑出研究獎

2014 中央研究院年輕學者研究著作獎

2014 科技部吳大猷先生紀念獎

實驗室成員展開收合
姓名 職稱 電話 Email 備註
蘇怡璇 研究員 02-2789-9511 yhsu@gate.sinica.edu.tw
范子霈 博士後 02-2789-9517 無
陳宜芝 研究助理 02-2789-9517 無
李函儒 研究助理 02-2789-9517 無
著作展開收合
  1. Chang, W.L. and Su, Y.H.* (2022) Zygotic hypoxia-inducible factor alpha regulates spicule elongation in the sea urchin embryo, Developmental Biology, 484, 63-74.
  2. Gil-Gálvez, A., Jiménez-Gancedo, S., Pérez-Posada, A., Franke, M., Acemel, R.D., Lin, C.Y., Chou, C., Su, Y.H., Yu, J.K., Bertrand, S., Schubert, M., Escrivá, H., Tena, J.J.*, Gómez-Skarmeta, J.L. (2022) Gain of gene regulatory network interconnectivity at the origin of vertebrates. Proceedings of the National Academy of Sciences of the United States of America, 119(11): e2114802119.
  3. Su, Y.H.* (2022) Dorsal-ventral axis formation in sea urchin embryos, Current Topics in Developmental Biology issue “Gene Regulatory Mechanisms in Development and Evolution: Insights from Echinoderms”, 146, 183-210  (invited book chapter).
  4. Lin, C.Y., Yu, J.K.*, Su, Y.H.* (2021) Evidence for BMP-mediated specification of primordial germ cells in the indirect-developing hemichordate, Evolution & Development, 23, 28-45
  5. Lin, C.Y., Lu, M.Y.J., Yue, J.X., Li, K.L., Le Petillon, Y., Yong, L.W., Chen, Y.H., Tsai, F.Y., Lyu, Y.F., Chen, C.Y., Hwang, S.P.L., Su, Y.H.*, Yu, J.K.* (2020) Single-blastomere transcriptome profiling reveals molecular asymmetry in the early amphioxus embryo, PLOS Genetics, 16(12): e1009294.
  6. Su, Y.H.*, Ota, K.G. (2020) Editorial: EvoDevo research in Asia, Evolution & Development, 22, 407-408.
  7. Yu, J.K.*, Su, Y.H. (2020) The evolution of the neural border and peripheral nervous system-Insights from invertebrate deuterostome animals (Book Chapter). Chapter 3 of Evolving Neural Crest Cells, edited by Brain Frank Eames, Danial Meulemans Medeiros, and Igor Adameyko, CRC Press.
  8. Li, Q., Zhang, P., Li, J., Yu, H., Zhan, X., Zhu, Y., Guo, Q., Tan, H., Lundholm, N., Garcia, L., Martin, M.D., Subirats, M.A., Su, Y.H., Ruiz-Trillo, I., Martindale, M.Q., Yu, J.K., Gilbert, M.T.P., Zhang, G., 2020. On the origin and evolution of RNA editing in metazoans. bioRxiv doi: https://doi.org/10.1101/2020.01.19.911685.
  9. Wang, Y.T., Tseng, T.L., Kuo, Y.C., Yu, J.K., Su, Y.H., Poss, K.D., Chen, C.H.* (2019) Genetic reprogramming of positional memory in a regenerating appendage. Current Biology, 29, 4193-4207.
  10. Coffman J.A.*, Su, Y.H.* (2019) Redox regulation of development and regeneration. Current Opinion in Genetics & Development: Developmental mechanisms, patterning and evolution, 57, 9-15 (invited review).
  11. Su, Y.H.*, Chen, Y.C., Ting, H.C., Fan, T.P., Lin, C.Y., Wang, K.T., Yu, J.K.* (2019) BMP controls dorsoventral and neural patterning in indirect-developing hemichordates providing insights into a possible origin of chordates. Proceedings of the National Academy of Sciences of the United States of America, 116, 12925-12932.
  12. Lin, C.Y., Oulhen, N., Wessel, G., Su, Y.H.* (2019) CRISPR/Cas9-mediated genome editing in sea urchins. Methods in Cell Biology, 151, 305-321 (Book Chapter).
  13. Campanale, J.P., Hamdoun, A., Wessel, G.M., Su, Y.H., Oulhen, N. (2019) Methods to label, isolate, and image sea urchin small micromeres, the primordial germ cells (PGCs). Methods in Cell Biology, 150, 269-292 (Book Chapter).
  14. Fan, T.P., Ting, H.C., Yu, J.K., Su, Y.H.* (2018) Reiterative use of FGF signaling in mesoderm development during embryogenesis and metamorphosis in the hemichordate Ptychodera flava. BMC Evolutionary Biology, 18, 120.
  15. Luttrell, S.M., Su, Y.H., Swalla, B.J.* (2018) Getting a head with Ptychodera flava larval regeneration. Biological Bulletin, 234, 152-164.
  16. Hu, M., Tseng, Y.C., Su, Y.H., Lein, E., Lee, H.G., Lee, J.R., Dupont, S., Stumpp, M.* (2017) Variability in larval gut pH regulation defines sensitivity to ocean acidification in six species of the Ambulacraria superphylum. Proceedings of the Royal Society B-Biological Sciences, 284, 20171066.
  17. Chang, W.L.#, Chang, Y.C.#, Lin, K.T., Li, H.R., Pai, C.Y., Chen, J.H., Su, Y.H.* (2017) Asymmetric distribution of hypoxia-inducible factor α regulates dorsoventral axis in the early sea urchin embryo. Development, 144, 2940-2950 (#contributed equally; cover and feature article).
  18. Su, Y.H.* and Yu, J.K.* (2017) EvoDevo: Changes in developmental controls underlying the evolution of animal body plans. Developmental Biology, 427, 177-178 (Editorial for the special issue).
  19. Cui, M. #, Lin, C.Y. #, and Su, Y.H.* (2017) Recent advances in functional perturbation and genome editing techniques in studying sea urchin development. Briefings in Functional Genomics, 16, 309-318 (invited review; #contributed equally).
  20. Chang, Y.C., Pai, C.Y., Chen, Y.C., Ting, H.C., Martinez, P., Telford, M.J., Yu, J.K., and Su, Y.H.* (2016) Regulatory circuit rewiring and functional divergence of the duplicate admp genes in dorsoventral axial patterning. Developmental Biology, 410, 108-118.
  21. Lin, C.Y. and Su, Y.H.* (2016) Genome editing in sea urchin embryos by using a CRISPR/Cas9 system. Developmental Biology, 409, 420-428.
  22. Lin, C.Y., Tung, C.H., Yu, J.K.*, Su, Y.H.* (2016) Reproductive periodicity, spawning induction, and larval metamorphosis of the hemichordate acorn worm Ptychodera flava. J Exp Zool B, 326, 47-60.
  23. Simakov, O.*, Kawashima, T., Marlétaz, F., Jenkins, J., Koyanagi, R., Mitros, T., Hisata, K., Bredeson, J., Shoguchi, E., Gyoja, F., Yue, J.X., Chen Y.C., Freeman, R.M. Jr, Sasaki, A., Hikosaka-Katayama, T., Sato, A., Fujie, M., Baughman, K.W., Levine, J., Gonzalez, P., Cameron, C., Fritzenwanker, J.H., Pani, A.M., Goto, H., Kanda, M., Arakaki, N., Yamasaki, S., Qu, J., Cree, A., Ding, Y., Dinh, H.H., Dugan, S., Holder, M., Jhangiani, S.N., Kovar, C.L., Lee, S.L., Lewis, L.R., Morton, D., Nazareth, L.V., Okwuonu, G., Santibanez, J., Chen, R., Richards, S., Muzny, D.M., Gillis, A., Peshkin, L., Wu, M., Humphreys, T., Su, Y.H., Putnam, N., Schmutz, J., Fujiyama, A., Yu, J.K., Tagawa, K., Worley, K.C., Kirschner, M.W., Lowe, C.J., Satoh, N.*, Rokhsar, D.S.*, Gerhart, J.* (2015) Hemichordate genomes and deuterostome origins. Nature, 527, 459-465.
  24. Andrikou, C., Pai, C.Y., Su, Y.H.*, Arnone, M.I.* (2015) Logics and properties of a genetic regulatory program that drives embryonic muscle development in an echinoderm. eLife, 4:e07343.
  25. Fan, T.P. and Su, Y.H.* (2015) FGF signaling repertoire of the indirect developing hemichordate Ptychodera flava. Marine Genomics, 24, 167-175.
  26. Stumpp, M., Hu, M.Y, Tseng, Y.C., Guh, Y.J., Chen Y.C., Yu, J.K., Su, Y.H., Hwang, P.P.* (2015) Evolution of extreme stomach pH in bilateria inferred from gastric alkalization mechanisms in basal deuterostomes. Scientific Reports, 5:10421.
  27. Satoh, N.*, Tagawa, K., Lowe, C., Yu, J.K., Kawashima, T., Takahashi, H., Ogasawara, M., Kirschner, M., Hisata, K., Su, Y.H., Gerhart, J. (2014) On a possible evolutionary link of the stomochord of hemichordates to pharyngeal organs of chordates. genesis, 52, 925-934.
  28. Chen, S.H., Li, K.L., Lu, I.H., Wang, Y.B., Tung, C.H., Ting, H.C., Lin, C.Y., Lin, C.Y.*, Su, Y.H.*, Yu, J.K.* (2014) Sequencing and analysis of the transcriptome of the acorn worm Ptychodera flava, an indirect developing hemichordate. Marine Genomics, 15, 35-43.
  29. Su Y.H.* (2014) Telling left from right: Left-right asymmetric controls in sea urchins. genesis, 52, 269-278 (invited review).
  30. Tung C.H. #, Cheng Y.R. #, Lin C.Y., Ho J.S., Yu J.K.*, Su Y.H.* (2014) A new copepod with transformed body plan and unique phylogenetic position parasitic in the acorn worm Ptychodera flava. Biological Bulletin, 226, 69-80 (#contributed equally).
  31. Ikuta T.#, Chen Y.C.#, Annunziata R., Ting H.C., Tung C.H., Koyanagi R., Tagawa K., Humphreys T., Fujiyama A., Saiga H., Satoh N., Yu J.K., Arnone M.I., Su Y.H.* (2013) Identification of an intact ParaHox cluster with temporal colinearity but residual spatial colinearity in the hemichordate Ptychodera flava. BMC Evolutionary Biology, 13, 129 (#contributed equally).
  32. Peterson K.J.*, Su Y.H., Arnone M.I., Swalla B.J., King, B. (2013) microRNAs support the monophyly of enteropneust hemichordates. J Experimental Zoology B, 320, 368-374.
  33. Ben-Tabou de-Leon S.*, Su Y.H.*, Lin, K.T., Li, E., Davidson, E.H.* (2013) Gene regulatory control in the sea urchin aboral ectoderm: Spatial initiation, signaling inputs, and cell fate lockdown. Developmental Biology, 374, 245-254.
  34. Luo Y.J., Su Y.H.* (2012) Opposing Nodal and BMP signals regulate left-right asymmetry in the sea urchin larva. PLoS Biology, 10(10), e1001402.
  35. Wu H.R., Chen Y.T., Su Y.H., Luo Y.J., Holland L.Z., Yu J.K.* (2011) Asymmetric localization of germline markers Vasa and Nanos during early development in the amphioxus Branchiostoma floridae. Developmental Biology, 353, 147-159.
  36. Chen J.H., Luo YJ, Su Y.H.* (2011) The dynamic gene expression patterns of transcription factors constituting the sea urchin aboral ectoderm gene regulatory network. Developmental Dynamics, 240, 410-421.
  37. Su Y.H.* (2009) Gene regulatory networks for ectoderm specification in sea urchin embryos. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, 1789, 261-267 (invited review).
  38. Su Y.H., Li E., Geiss G.K., Longabaugh W.J., Kramer A, Davidson E.H.* (2009) A perturbation model of the gene regulatory network for oral and aboral ectoderm specification in the sea urchin embryo. Developmental Biology, 329, 410-421. 
  39. Nam J, Su Y.H., Lee P.Y., Robertson A.J., Coffman J.A., Davidson E.H.* (2007) Cis-regulatory control of the nodal gene, initiator of the sea urchin oral ectoderm gene network. Developmental Biology, 306, 860-869.
  40. Churamani D, Boulware M.J., Geach T.J., Martin A.C., Moy G.W., Su Y.H., Vacquier V.D., Marchant J.S., Dale L, Patel S.* (2007) Molecular characterization of a novel intracellular ADP-ribosyl cyclase. PLoS One, 2(8): e797.
  41. Roux, M.M., Townley, I.K., Raisch, M., Reade, A., Bradham, C., Humphreys, G., Gunaratne, H.J., Killian, C.E., Moy, G., Su, Y.H., Ettensohn, C.A., Wilt, F., Vacquier, V.D., Burke, R.D., Wessel, G., and Foltz, K.R.* (2006) A functional genomic and proteomic perspective of sea urchin calcium signaling and egg activation. Dev Biol., 300, 416-433.
  42. Sea Urchin Genome Sequencing Consortium* (2006) The genome of the sea urchin Strongylocentrotus purpuratus. Science, 314, 941-952.
  43. Su, Y.H. and Vacquier, V.D.* (2006) Cyclic GMP-specific phosphodiesterase-5 regulates motility of sea urchin spermatozoa. Mol. Biol. Cell, 17, 114-121.
  44. Su, Y.H.*, Chen, S.H, Zhou, H., and Vacquier, V.D. (2005) Tandem mass spectrometry identifies proteins phosphorylated by cyclic AMP-dependent protein kinase when sea urchin sperm undergo the acrosome reaction. Developmental Biology, 285, 116-125.
  45. Su, Y.H. and Vacquier, V.D.* (2002) A flagellar K+-dependent Na+/Ca2+ exchanger keeps Ca2+ low in sea urchin spermatozoa. Proc. Natl. Acad. Sci. USA, 99, 6743-6748.
  46. Jang, C.W., Chen C.H., Chen, C.C., Chen, Jy.J., Su, Y.H., and Chen, R.H.* (2002) TGF-beta induces apoptosis through Smad-mediated expression of DAP-kinase. Nature Cell Biol., 4, 51-58.
  47. Tsai, Y.T.#, Su, Y.H.#, Fang, S.S., Huang, T.N., Qiu, Y., Jou, Y.S., Shih, H.M., Kung, H.J., and Chen, R.H.* (2000) Etk, a Btk family tyrosine kinase, mediates cellular transformation by linking Src to STAT3 activation. Mol. Cell Biol., 20, 2043-2054 (#contributed equally).
  48. Chen, R.H., Chang, M.C., Su, Y.H., Tsai, Y.T., and Kuo, M.L.* (1999) Interleukin-6 inhibits transforming growth factor-b-induced apoptosis through the phosphatidylinositol 3-kinase/Akt and signal transducers and activators of transcription 3 pathways. J. Biol. Chem., 274, 23013-23019.
  49. Hwang, S.P.L.*, Lin, Y.C., Su, Y.H., and Chen, C.P. (1999) Accelerated development of embryonic spicule and micromere-derived primary mesenchyme cell culture of the sea urchin Stomopneustes variolaris (Lamarck). Invertebr. Reprod. Dev., 35, 89-93.
  50. Chen, R.H.*, Su, Y.H., Chuang, R.L.C., and Chang, T.Y. (1998) Suppression of transforming growth factor-b-induced apoptosis through a phosphatidylinositol 3-kinase/Akt-dependent pathway. Oncogene, 17,1959-1968.