神經迴路及行為實驗室
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- 專長:Developmental Neurobiology, Neuronal circuitry, Insect Behavior
- 信箱:yhchou@gate.sinica.edu.tw
- 電話:02-2789-9540
- 位置:R202/ICOB
如同莊子與惠子的魚樂之辯,當面對同一顆蘋果,我們也無從得知自己聞到的蘋果味道是否與旁人聞到的完全一樣。事實是,雖然每個人的大腦都經由相同的發育機轉而架構、成熟,當面對相同的環境刺激,每個個體接收訊息的程度並不會完全相同,而這種差異最終會反映在我們對周遭環境的判讀及行為反應。我們實驗室即對神經差異性(Neuronal variability)的形成及其對個體行為的影響有興趣。
果蠅利用與哺乳動物相似的嗅覺神經系統來分辨環境中的氣味分子(Odorant),如其天敵、食物、配偶的氣味,以決定下一刻要作何反應。果蠅的嗅神經細胞 (Olfactory receptor neuron; ORN)一旦接收環境中的氣味分子,會將訊息傳遞到腦中的嗅葉(Antennal lobe; AL)。在此,二級神經元(Projection neuron; PN)會接收訊息並將其傳遞到不同的腦區域中心,如 mushroom body (MB)及lateral horn (LH)去做進一步的訊息整合及判讀。然而在嗅球周圍還有二群區域中介神經元 (Local interneuron; LN),他們在氣味分子訊息被傳遞到二級神經元之前,即先將這些訊息整合、變化及轉譯。
果蠅的嗅覺神經系統相較於哺乳類動物簡單,但卻可以接收並處理龐大、複雜的訊息而決定其多樣、精細的行為反應。我們之前發現,果蠅嗅覺神經系統的區域中介神經元在形態、電生理反應、神經連結及功能等方面具有高度的神經差異性具有相當複雜的多樣性(Diversity)及差異性(Variability),但對於這些細胞如何在發育過程中及個體成熟後達到其多樣性及差異性一無所知。因此我們希望經由系統性地研究區域中介神經元的發育、其如何選擇與特定的嗅覺神經元及二級神經元形成獨特的連結、以及其差異性對個體行為的影響,來瞭解不同個體大腦中神經迴路的差異性是如何造成的、參與的分子機制為何、以及其如何影響我們對外界環境的判讀。
目前我們實驗室進行中的研究包括 :

(1)系統性的研究不同類型區域中介神經元的發育:在過去幾年,我們實驗室先建立了果蠅嗅覺區域中介神經元的標定工具及其發育圖譜 (圖ㄧ)。另外,我們也完成了三項大規模的遺傳篩選,以尋找可能調控這些發育過程的基因。目前我們正利用這些工具及圖譜,進一步去探討區域中介神經元如何經由不同的分子調控機制而建立他們的多樣性。
(2)探討神經差異性的起源分子機制及其對行為的影響:我們最近證明單一區域中介神經元可呈現至少849種不同的型態,並影響神經迴路的連結及果蠅的攝食行為。目前我們正利用實驗室建立的、標定不同單一特定區域中介神經元的系統,以探討不同區域中介神經元差異性的起源及其對果蠅行為的影響。

(3)利用我們收集到的大量行為實驗數據,建立數學模擬系統、以暸解區域中介神經元如何影響嗅覺訊息在神經迴路的變化及傳遞:我們已建立了數套結合數學運算及機器學習的果蠅行為偵測系統(如圖二)。目前正經由結合鈣離子影像偵測、光遺傳系統、我們建立的果蠅行為偵測系統以及數學模型,以探討特定嗅覺神經元的差異性如何影響神經訊號在迴路之間的動態傳遞以及對個體行為的影響。
- 2019 - Editorial board, Journal of Neurogenetics
- 2016 Selected as eLife early-career reviewer
- 2013 Career Development Award (Academia Sinica)
- 2012 Initial Employment Academic Research Grants (Academia Sinica)
- 2003 Chien-Tien Hsu Outstanding Graduate Dissertation Award (The Chinese Society of Cell and Molecular Biology)
- 2003 Outstanding Students Conference Travel Grant (Foundation for the Advancement of Outstanding Scholarship)
姓名 | 職稱 | 電話 | 備註 | |
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周雅惠 | 副研究員 | 02-27899540 | yhchou@gate.sinica.edu.tw | Aug 2011~ |
Viet-Dai Dang | 博士後研究員 | 02-27899509 | 無 | Feb. 2021 ~ |
張顥馨 | 博士班研究生 | 02-27899509 | s0170543@gate.sinica.edu.tw | Aug. 2016~ |
謝忻倜 | 研究助理 | 02-27899509 | 無 | Aug 2019~ |
沈軒維 | 研究助理 | 02-27899509 | 無 | Oct. 2019~ |
Michael Panganiban | 研究助理 | 02-27899509 | 無 | July 2020~ |
陳劭杰 | 研究助理 | 02-27899509 | ermae@gate.sinica.edu.tw | Jan 2021~ |
潘映潔 | 行政助理 | 02-27899509 | 無 | Jan. 2022 ~ |
周星賢 | 專科醫師 | 02-27899509 | b85401053@ntu.edu.tw | Aug 2011~ |
王桂枝 | 助理 | 02-27899509 | 無 | Jan 2012~ |
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- Julia Elia Manoim-Wolkovitz; Tal Camchy; Eyal Rozenfeld; Hao-Hsin Chang; Hadas Lerner; Ya-Hui Chou; Ran Darshan; Moshe Parnas. Non-linear High Activity Neuronal Excitation Enhances Odor Discrimination. (Under revision in Current Biology)
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- Tsai, K.T., Chou, Y.H.* (2022) Drosophila as a Model to Explore Individuality. In: Yamamoto, D. (eds) Behavioral Neurogenetics. Neuromethods, v181, 223-255. Humana, New York, NY. Springer Protocols. DOI: 10.1007/978-1-0716-2321-3_13
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- Chou, Y.H.*, Yang, C.J., Huang, H.W., Liou, N.F., Panganiban, M.R., Luginbuhl, D., Yin, Y., Taisz, I., Liang, L., Jefferis, G.S.X.E., Luo, L. (2022) Mating-driven Variability in Olfactory Local Interneuron Wiring. Science Advances. 8, eabm7723. DOI: 10.1126/sciadv.abm7723
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- Tsai, K.T., Chou, Y.H.* (2019) Random walk revisited: quantification and comparative analysis of Drosophila walking trajectories. iScience 19, 1145-1159. DOI: 10.1016/j.isci.2019.08.054.
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- Tsai, K.T., Liou, N.F. and Chou, Y.H.* (2019). Quantitatively analyzing morphological phenotypes of neurons and neuropils through three-dimensional reconstruction of Drosophila brains. Bio-101. e1010290. DOI: 10.21769/BioProtoc.10101290. (in Chinese)
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- Chang, H. H. and Chou, Y. H.* (2019). Labeling single cells by Mosaic analysis with a repressible maker (MARCM) in Drosophila nervous system. Bio-101. e1010289. DOI:10.21769/BioProtoc.10101289. (in Chinese)
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- Yang, C.J., Tsai, K.T., Liou, N.F., Chou, Y.H.* (2019) Interneuron diversity: toward a better understanding of interneuron development in the olfactory system. J. Experimental Neuroscience. 13, 1-5. (Invited review). DOI:10.1177/1179069519826056
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- Chen, Y.J.#, Chang, H.H.#, Lin, S.H.#, Lin, T.Y., Wu, T.H., Lin, H.J., Liou, N.F., Yang, C.J., Chen, Y.T., Chang, K.H., Li,C.Y., Chou, Y.H.* (2019) Differential efficacy of genetically swapping GAL4. J. Neurogenetics. 33, 52-63. DOI:10.1080/01677063.2018.1564289
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Liou, N.F.#, Lin, S.H.#, Chen, Y.J.#, Tsai, K.T.&, Yang, C.J.&, Lin, T.Y., Wu, T.H., Lin, H.J., Chen, Y.T., Gohl, D.M., Silies, M., Chou, Y.H.* (2018) Diverse populations of local interneurons integrate into the Drosophila adult olfactory circuit. Nature Communications 9, 2232. DOI:10.1038/s41467-018-04675-x
Recommended by F1000Prime. Hiesinger P:F1000Prime, 10.3410 (Dec. 2018) 10.3410/f.733424303.793554440
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- Tsai, K.T., Hu, C.K., Li, K.W., Hwang, W.L., Chou, Y.H.* (2018) Circuit variability interacts with excitatory-inhibitory diversity of interneurons to regulate network encoding capacity. Scientific Reports 8, 8027. DOI:10.1038/s41598-018-26286-8
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- Hsia, E.Y.C., Zhang, Y., Tran, H.S., Lim, A., Chou, Y.H., Lan, G., Beachy, P.A., Zheng, X. (2017) Hedgehog mediate degradation of Ihog adhesion peoteins modulates cell segragation in Drosophila wing imaginal discs. Nature Communications 8, 1275. DOI:10.1038/s41467-017-01364-z
- 12
- Wu, B., Li J., Chou, Y.H., Luginbuhl D., and Luo L. (2017) Fibroblast growth factor signaling instructs ensheathing glia warpping of Drosophila olfactory glomeruli. Proceedings of the National Academy of Sciences USA 114, 7505-7512. DOI:10.1073/pnas.1706533114
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- Sweeney, L.B., Chou, Y.H.#, Wu, Z.#, Joo W.#, Komiyama, T., Potter, C.J., Kolodkin, A., Garcia, K.C., and Luo, L. (2011) Secreted Semaphorins from degenerating larval ORN axons direct adult projection neuron dendrite targeting. Neuron 72, 734-747. DOI:10.1016/j.neuron.2011.09.026
# equal authorship
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- Chou, Y.H., Zheng, X., Beachy, P.A. and Luo, L. (2010) Patterning axon targeting of olfactory receptor neurons by coupled Hedgehog signaling at two distinct steps. Cell 142, 954-966. DOI:10.1016/j.cell.2010.08.015
Featured in Nat. Rev. Neurosci, Research Highlights. (Nov. 2010) DOI:10.1038/nrn2940
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- Chou, Y.H.#, Spletter, M.L.#, Yaksi, E.#, Leong, J.C.S., Wilson, R.I. and Luo, L. (2010) Diversity and wiring variability of olfactory local interneurons in the Drosophila antennal lobe. Nature Neuroscience 13, 439-449. DOI:10.1038/nn.2489
# equal authorshipFeatured on the cover
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- Sweeney, L.B., Couto, A.#, Chou, Y.H.#, Berdnik, D., Dickson, B.J., Luo, L., and Komiyama, T. (2007). Temporal target restriction of olfactory receptor neurons by Semaphorin-1a/PlexinA-mediated axon-axon interactions. Neuron 53, 185-200. DOI:10.1016/j.neuron.2006.12.022
# equal authorshipPreview by Greg J. Bashaw DOI:10.1016/j.neuron.2007.01.002
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- Chou, Y.H. and Chien, C.T. (2002). Scabrous controls ommatidial rotation in the Drosophila compound eye. Developmental Cell 3, 839-850. https://doi.org/10.1016/S1534-5807(02)00362-3
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- Hsu, S.L., Chen, M.C., Chou, Y.H., Hwang, G.Y., and Yin, S.C. (1999). Induction of P21CIP1/Waf1 and activation of p34cdc2 involved in retinoic acid-induced apoptosis in human hepatoma Hep3B cells. Experimental Cell Resesrach 248, 87-96. DOI:10.1006/excr.1999.4397
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- Hsu, S.L.*, Chou, Y.H., Yin, S.C., and Liu, J.Y. (1998). Differential effects of phorbol ester on growth and protein kinase C isoenzyme regulation in human hepatoma Hep3B cells. Biochemical Journal 333, 57-64. DOI: 10.1042/bj3330057.
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- Chou, Y.H. and Yung, B.Y. M.* (1995). Cell cycle phase-dependent changes of localization and oligomerization states of nucleophosmin/B23. Biochemical and Biophysical Research Communications 217, 313-325. DOI: 10.1006/bbrc.1995.2779.