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研究人員｜中央研究院 細胞與個體生物學研究所

研究人員
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側邊選單開關 研究人員
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曾庸哲老師實驗室

  • 曾庸哲Yung-Che Tseng
    副研究員Associate Research Fellow
    • 專長：Marine ecophysiology, Evolutionary and comparative physiology, Systeomics
    • 信箱：yctseng@gate.sinica.edu.tw
    • 電話：03-9880-544 ext.14
    • 網站： 曾庸哲老師實驗室
    • 位置：B106/MRS
友善列印
實驗室簡介展開收合

研究方向

       本實驗室致力於探討水生生物面臨全球氣候變遷緊迫（海洋酸化及溫度變化），以及極端環境因子的波動衝擊下，水生生物如何進行有效地進行系統性的生理調節以適應變動的環境。實驗室運用各式「體學」研究策略（次世代基因體學、代謝體學、甲基化體學、總體基因體學）、感官電生理、即時生命現象與行為表徵檢測、以及表觀遺傳等研究方法，並結合臺灣四面環海的絕佳海洋生物學研究優勢，分別運用臺灣海域的頭足類動物（軟絲、花枝、章魚）、硬骨魚（吳郭魚、青鱂魚）、以及甲殼類動物（烏龜怪方蟹、大閘蟹）為研究物種，進行綜合性比較生理學研究。以求突破過往海洋生物研究的知識與傳統，準確且深入地探討海洋動物生態學表徵背後的生理學意義。

1. 烏龜怪方蟹耐酸耐毒機制的共生體學研究
熱泉系統支持著獨特的生態系統，其中包含了從微生物到脊椎動物豐富的生物多樣性。為了要在如此極端的環境生存，生物發展出特殊的形態和生理機制，以適應高溫、高硫、低氧、高二氧化碳和低酸鹼值的棲地。
    臺灣北方的龜山島的淺海熱泉即是這類生物的代表棲地。龜山島熱泉的是一個高溫(76-116℃)、極度酸化(pH 1.5-2.5)的淺海熱泉系統；此處的排水含有大量的硫化物。在如此特殊的環境中，只有少數物種可以生存。例如在噴口附近的烏龜怪方蟹(Xenograpsus testudinatus)，是唯一被記錄生存於此的後生動物。
    對甲殼類動物而言，鰓是參與細胞外離子與酸鹼平衡的主要器官，本團隊最近的研究中，我們找到烏龜怪方蟹鰓上主要的酸價與硫的排除通道，這些穿膜通道協助維持烏龜怪方蟹的細胞生理恆定。此外，龜山島熱泉對生物的生理挑戰不僅是酸鹼調節，同時也包括了如何克服環境硫化氫的毒性。我們認為，在熱泉系統中的甲殼類應該具有比其他物種更好的控制硫化物濃度能力，在其鰓上，更存在有特殊菌相，與烏龜怪方蟹形成特殊的細胞內共生關係。
    極端環境中的共生現象，對棲息於此棲地的生物而言，不僅是一個物種演化的現象，在物種與生態的永續維持也至關重要。

2. 海洋酸化進程中，海洋生物的跨世代反應
工業革命後，人類活動造成大氣中二氧化碳濃度逐漸上升，當二氧化碳溶入海水會解離出的碳酸(氫)根離子與氫離子，造成海水的pH值下降，這個現象稱為「海洋酸化」。目前關於海洋酸化議題的研究明確指出：海水pH值下降會影響海洋生物的多樣性以及海洋生物的生理恆定，因此海洋酸化被認為是一個重要的全球性環境議題。
    近年針對海洋生物反應的相關研究已發現：對於環境酸化波動具備耐受能力的三類動物：硬骨魚類、頭足類、甲殼類螃蟹，在平衡體內酸鹼恆定的生理機制上，具有趨同調適的特徵。此外，它們在跨世代環境適應過程中，生理與行為等表徵亦會產生表觀遺傳特性的修飾機制。藉由比較不同海洋生物的綜合性研究，我們得以瞭解未來海洋生物可能的分佈、反應、與後續的生態鏈連鎖效應，因此，這些具有重要的預測性生物指標意義。

3. 水生動物耐寒性狀的表觀遺傳性、基因型及系統生物學研究
低溫緊迫已知會使誘發水生動物複雜的基因相變化及生化反應，並在一些重要器官強化粒線體的活性以提高代謝機制。然而粒線體提升呼吸作用的同時可能會導致生物體的傷害，包括活性氧化物質（ROS）的形成累積、粒線體內膜氫離子的溢漏增加、以及脂質過氧化反應的產生。其中粒線體ROS過量累積已知會造成細胞面臨氧化壓力。因此，我們透過了解水生動物適應性代謝調節、轉錄體與代謝體資料庫建構、以及行為表徵的模式建立，綜合探討全球氣候變遷導致的溫度異常下，水生動物生理調節的變化，並進行細胞抗氧化相關機制的觀察。這些發現對於往後進一步研究並發展出應用於水生動物養殖產業的實際可行的策略，提供了重要的素材。

實驗室成員展開收合
姓名 職稱 電話 Email 備註
曾庸哲 助研究員 +886-(0)3-9880544#14 yctseng@gate.sinica.edu.tw
顧穎傑 博士後研究員 +886-(0)3-9880544#24 yingjeyguh@gmail.com
Garett J. P. Allen 博士後研究員 ++886-(0)3-9880544#18 alleng3@myumanitoba.ca
關寶龍 研究助理 +886-(0)3-9880544#18 jio47123456@yahoo.com.hk
孫翊仁 研究助理 +886-(0)3-9880544#24 elainesun442@gmail.com
Mikyla T. Nash 研究助理 +886-(0)3-9880544#18 MikylaTNash@outlook.com
邱翎 博士班研究生 +886-(0)3-9880544#24 r08b22038@ntu.edu.tw
劉姿延 博士班研究生 +886-(0)3-9880544#24 rw6526@gmail.com
著作展開收合

(A) Book Chapter

Marian Y. Hu and Tseng. Y. C.# "Acid–Base Regulation and Ammonia Excretion in Cephalopods: An Ontogenetic Overview." Acid-Base Balance and Nitrogen Excretion in Invertebrates. Springer International Publishing, 2017. (pp. 275-298). 

(B)Peer-Reviewed Publications (International Journal)

  1. Chou, P. H., Hu, M. Y., Guh, Y. J., Wu, G. C., Yang, S. H., Tandon, K., Shao, Y. T., Lin, L. Y., Chen, C., Tseng, K. Y., Wang, M.C., Chen, C. M., Han, B. C., Lin, C. C., Tang, S. L., Jeng, M. S., Chang, C. F., Tseng, Y. C.# Cellular mechanisms underlying extraordinary sulfide tolerance in a crustacean holobiont from hydrothermal vents. Proc. R. Soc. B Biol. Sci., 290, 20221973, 2023.
  2. Lin, M. W., Kuan, P. L., Sung, P. H., Tseng, Y. C.# Metabolic trade-offs associated with homeostatic adjustments in pelagic and benthic cephalopods: comparative evaluations of NH4+/H+ transport machinery in gills. Front. Mar. Sci., 9:971764, 2022.
  3. Tseng, Y. C.#, Yan, J. J., Furukawa, F., Chen, R. D., Liu, T. Y., Tang, Y. H., Hwang, P. P. Teleostean fishes may have developed an efficient Na+ uptake for adaptation to the freshwater system. Front. Physiol., 13:947958, 2022.
  4. Chiu, L., Wang, M. C., Tseng, K. Y., Wei, C. L., Lin, H. T. Yang, S. H., Tseng, Y. C.# Shallow-water hydrothermal vent system as an extreme proxy for discovery of microbiome significance in a crustacean holobiont. Front. Mar. Sci., 9, 976255, 2022.
  5. Kuan, P. L., You, J.Y., Wu, G.C., Tseng, Y. C.# Temperature increases induce metabolic adjustments in the early developmental stages of bigfin reef squid (Sepioteuthis lessoniana). Sci. Total Environ., 844, 156962, 2022.
  6. Wang, M. C., Furukawa, F., Wang, C. W., Peng, H. W., Lin, C. C., Lin, T. H., Tseng, Y. C.#. Multigenerational inspections of environmental thermal perturbations promote metabolic trade-offs in developmental stages of tropical fish. Environ. Pollut., 308, 119605, 2022.
  7. Wang, M. C., Hsu, M. T., Lin, C. C., Hsu, S. C., Chen, R. D., Lee, L. R., Chou, Y, L., Tseng, H. P., Furukawa, F., Hwang, S. P. L., Hwang P. P., Tseng. Y. C.# Adaptive metabolic provision in a thermostabilized system: those transgenerational trade-off implications from tropical tilapia. Sci. Total. Environ., 806, 150672, 2022.
  8. Quijada-Rodriguez, A. R., Kuan, P. L., Sung, P. H., Hsu, M. T., Allen, G. J. P., Hwang, P. P., Tseng, Y. C.#, Weihrauch, D. Anthropogenic CO2-mediated freshwater acidification limits survival, calcification, metabolism, and behaviour in stress-tolerant freshwater crustaceans. Biogeosciences, 18, 6287–6300, 2021.
  9. Huang, P. C., Liu, T. Y., Hu, M. Y., Casties, I., Tseng, Y. C.# Energy and nitrogenous waste from glutamate/glutamine catabolism facilitates acute osmotic adjustment in non-neuroectodermal branchial cells. Sci. Rep., 10, 9460, 2020.
  10. Wang, M .C., Wang, Y. C., Peng. H. W., Hseu, J. R., Wu, G. C., Chang, C. F., Tseng. Y. C. (2020) Resveratrol induces expression of metabolic and antioxidant machinery and protects tilapia under cold stress. Int J Mol Sci, 21, e3338. 
  11. Tseng, Y. C.., Yan, J. J., Furukawa, F., Hwang, P.P . (2020) Did acidic stress resistance in vertebrates evolve as Na+/H+ exchanger-mediated ammonia excretion in fish? Bioessays, 42, e1900161.
  12. Allen, G. J. P., Kuan, P. L., Tseng, Y. C., Hwang, P. P., Quijada-Rodriguez, A. R., Weihrauch, D. (2020) Specialized adaptations allow vent-endemic crabs (Xenograpsus testudinatus) to thrive under extreme environmental hypercapnia. Sci Rep, 10, 11720. 
  13. Yan, J. J., Lee, Y. C., Chou, Y. L., Tseng, Y. C., Hwang, P. P. (2020) Insulin-like growth factor 1 regulates salt secretion machinery in fish under acute salinity stress. J Endocrinol. JOE-20-0053.
  14. Li, C. Y.*, Tseng, Y. C.*, Chen, Y. J., Yang, Y., Hsu, Y. (2020) Personality and physiological traits predict contest interactions in Kryptolebias marmoratus. Behav Processes, 173, 104079. (* Equal Contribution) 
  15. Lee, H. G., Stumpp, M., Yan, J. J., Tseng, Y. C., Heinzel, S., Hu, M. Y. (2019) Tipping points of gastric pH regulation and energetics in the sea urchin larva exposed to CO2 induced seawater acidification. Comp Biochem Physiol A Mol Integr Physiol, 234, 87-97.
  16. Shao, Y. T., Chang, S. Y., Chang, H. Y., Tseng, Y. C., Shao, K. T. (2018). Largescale mullet (Planiliza macrolepis) can recover from thermal pollution-induced malformations. PLoS ONE 13(11): e0208005.
  17. Narita, Y., Tsutiya, A., Nakano, Y., Ashitomi, M., Sato, K., Hosono, K., Kaneko, T., Chen, R. D., Lee, J. R., Tseng, T. C., Hwang, P. P., Otani-Kaneko, R. (2018). Androgen induced cellular proliferation, neurogenesis, and generation of GnRH3 neurons in the brain of mature female Mozambique tilapia. Sci Rep. 8, 16855.
  18. Shiao, J. C., Shirai, K., Tanaka, K., Takahata, N., Sano, Y., Hsiao, S. Y., Lee, D. C., Tseng, Y. C. (2018). Assimilation of nitrogen and carbon isotopes from fish diets to otoliths as measured by nanoscale secondary ion mass spectrometry. Rapid Commun Mass Spectrom, 32, 1250-1256, 2018.
  19. Hu, M. Y., Tseng, Y. C., Su, Y. H., Lein, E., Lee, J. H., Dupont, S., Stumpp, M. Variability in larval gut pH regulation defines sensitivity to ocean acidification in six species of the Ambulacraria superphylum. Proc R Soc Lond B Biol Sci, 287, 20171066, 2017.
  20. Wei, C. A., Lin, T. H., Che, R. D., Tseng, Y. C., Shao, Y. T. The effects of continuously acoustical stress on cortisol in milkfish (Chanos chanos). Gen Comp Endocrinol, 257, 227-234, 2017.
  21. Hu, M. Y., Sung, P. H., Guh, Y. J., Lee, J. R., Hwang, P. P., Weihrauch, D., Tseng, Y. C.# Perfused gills reveal fundamental principles of pH regulation and ammonia homeostasis in the cephalopod Octopus vulgaris. Front Physiol, 8, 162, 2017. 
  22. Weaver, R., Hill, G. E., Kuan, P. L., Tseng, Y. C.# Copper exposure reduces production of red carotenoids in a marine copepod. Ecol Indic, 70, 393-400, 2016.
  23. Hu, M. Y., Michael, K., Kreiss, C. M., Stumpp, M., Dupont, S, Tseng, Y. C.#, Lucassen, M. Temperature modulates the effects of ocean acidification on intestinal ion transport in Atlantic cod, Gadus morhua. Front Physiol, 7, 198, 2016.
  24. Hu, M. Y., Guh, Y. J., Shao, Y. T., Kuan, P. L., Chen, G. L., Lee, J. R., Jeng, M. S., Tseng, Y. C.#. Strong ion regulatory abilities enable the crab Xenograpsus testudinatus to inhabit highly acidified marine vent systems. Front Physiol 7, 14, 2016. 
  25. Wang, Y. F., Yan, J. J., Tseng, Y. C. , Chen, R. D., Hwang, P. P. Molecular physiology of an extra-renal Cl- uptake mechanism for body fluid Cl- homeostasis. Int J Biol Sci 11(10), 1190-1203, 2015.
  26. Hu, M. Y., Hwang, P. P., Tseng, Y. C.# Recent advances in understanding trans-epithelial acid-base regulation and excretion mechanisms in cephalopods. Tissue Barriers 3(4), e1064196.
  27. Stumpp, M., Hu, M. Y., Tseng, Y. C., Guh, Y. J., Chen, Y. C., Su, Y. H., Yu, J. K., Hwang, P. P. Evolution of extreme pH in bilaterian digestive systems inferred from gastric alkalization mechanisms in basal deuterostomes. Sci Rep 5, 10421, 2015.
  28. Furukawa, F., Tseng, Y. C., Liu, S. T., Chou, Y. L., Lin, C. C., Sung, P. H., Uchida, K., Lin, L. Y., Hwang, P. P. Induction of phosphoenolpyruvate carboxykinase (PEPCK) during acute acidosis and its role in acid secretion by V-ATPase-expressing ionocytes. Int J Biol Sci 16(2), 712-725, 2015.
  29. Shao, Y. T., Tseng, Y. C.#, Chang, C. H., Yan, H. Y, Hwang, P. P., Borg, B. GnRH mRNA levels in male three-spined sticklebacks, Gasterosteus aculeatus, under different reproductive conditions. Comp Biochem Physiol A Mol Integr Physiol 180, 6-17, 2015. 
  30. Hu, M. Y., Guh, Y. J., Stumpp, M., Lee, J. R., Chen, R. D., Sung, P. H., Chen, Y. C., Hwang, P. P. and Tseng, Y. C.#. Branchial NH4+-dependent acid-base transport mechanisms and energy metabolism of squid (Sepioteuthis lessoniana) affected by seawater acidification. Front Zool 11, 55, 2014.
  31. Tseng, Y. C., Liu, S. T., Hu, M. Y., Chen, R. D., Lee, J. R., and Hwang, P. P. Brain functioning under acute hypothermic stress supported by dynamic monocarboxylate utilization and transport in ectothermic fish. Front Zool 11, 53, 2014. 
  32. Hsu, H. H., Lin, L. Y., Tseng, Y. C., Horng, J. L., and Hwang, P. P. A new model for fish ion regulation: Identification of ionocytes in freshwater- and seawater-acclimated medaka (Oryzias latipes). Cell Tissue Res 357(1), 225-243, 2014. 
  33. Guh, Y. J., Tseng, Y. C., Yang, C. Y., and Hwang, P. P. Endothelin-1 regulates H+-ATPase-dependent transepithelial H+ secretion in zebrafish. Endocrinology 155(5), 1728-1737, 2014. 
  34. Lee, L. C., Wang, Y. T., Wu., Y. R., Soong, B. W., Tseng, Y. C., Chen, C. M., Lee, G. J. Downregulation of proteins involved in the endoplasmic reticulum stress response and Nrf2-ARE signaling in lymphoblastoid cells of spinocerebellar ataxia type 17. J Neural Transm 121(6), 301-610, 2014.
  35. Chen, C. M., Wu, C. H., Hsieh, C. H., Lin, C. H., Chen, I. C., Chen, Y. C., Lee, L. C., Lee, C. M., Tseng, Y. C., Lee, G. J., and Wu, Y. R. HTRA2 variations in Taiwanese Parkinson’s disease. J Neural Transm 121(5), 491-198, 2014.
  36. Hu, M. Y., Lee, J. R., Lin, L. Y., Shih, T. H., Stumpp,M., Lee, M. F., Hwang, P. P. and Tseng, Y. C.#. Development in a naturally acidified environment: Na+/H+-exchanger 3-based proton secretion leads to CO2 tolerance in cephalopod embryos. Front Zool 10(1), 51, 2013.
  37. Tseng, Y. C., Hu, M. Y., Stumpp, M., Lin, L. Y., Melzner, F., and Hwang, P. P. CO2-driven seawater acidification differentially affects development and molecular plasticity along life history of fish (Oryzias latipes). Comp Biochem Physiol A Mol Integr Physiol 165(2), 119-130, 2013. 
  38. Tseng, Y. C., Kao, Z. J., Liu, S. T., Chen, R. D., and Hwang, P. P. Spatial expression and functional flexibility of monocarboxylate transporter isoforms in the zebrafish brain. Comp Biochem Physiol A Mol Integr Physiol 165(2), 106-118, 2013. 
  39. Shao, Y. T., Tseng, Y. C., Trombley, S., Hwang, P. P., Schmitz, M., and Borg, B. Schistocephalus solidus infections increase gonadotropins and gonadotropin releasing hormone (GnRH3) mRNA levels in the three-spined stickleback, Gasterosteus aculeatus. Parasitol Int 61(3), 470-474, 2012. 
  40. Hu, M. Y.*, Tseng, Y. C.*, Lin, L. Y., Chen, P. Y., Charmantier-Daures, M., Hwang, P. P., and Melzner, F. New insights into ion regulation of cephalopod molluscs: a role of epidermal ionocytes in acid-base regulation during embryogenesis. Am J Physiol Regul Integr Comp Physiol 301(6), R1700-R1709, 2011. (* Equal Contribution)
  41. Tseng, Y. C., Chen, R. D., Lucassen, M., Schmidt, M. M., Abele, D., and Hwang, P. P. Exploring uncoupling proteins and anti-oxidative stress mechanisms under acute cold exposure in brains of fish. PLoS ONE 6(3), e18180, 2011. 
  42. Hu, M. Y., Tseng, Y. C., Stumpp, M., Gutowska, M. A., Kiko, R., Lucassen, M., and Melzner, F. Elevated seawater pCO2 differentially affects branchial acid-base transporters over the course of development in the cephalopod Sepia officinalis. Am J Physiol Regul Integr Comp Physiol 300(5), R1100-R1114, 2011. 
  43. Tseng, Y. C., Lee, J. R., Lee, S. J., and Hwang, P. P. Functional analysis of glucose transporter 1a, -6, and -13.1 expressed in epithelial cells. Am J Physiol Regul Integr Comp Physiol 300(2), R321-R329, 2011.
  44. Lafont, A. G., Wang, Y. F., Chen, G. D., Liao, B. K., Tseng, Y. C., Hwang, C. J., and Hwang, P. P. Involvement of calcitonin and its specific receptor in the control of calcium regulating-genes and calcium homeostasis in zebrafish (Danio rerio). J Bone Miner Res 26(5), 1072-1083, 2011.
  45. Cruz, S., Tseng, Y. C., Kaiya, H., and Hwang, P. P. Ghrelin affects carbohydrate-glycogen metabolism via insulin inhibition and glucagon stimulation in the zebrafish (Danio rerio) brain. Comp Biochem Physiol A Mol Integr Physiol 156(2), 190-200, 2010.
  46. Tseng, Y. C., Chen, R. D., Lee, J. R., Liu, S. T., Lee, S. J., and Hwang, P. P. Specific expression and regulation of glucose transporters in zebrafish ionocytes. Am J Physiol Regul Integr Comp Physiol 297(2), R275-R290, 2009. (a congratulation letter from the editor) 
  47. Wang, Y. F., Tseng, Y. C., Yan, J. J., Hiroi, J., and Hwang, P. P. Role of SLC12A10.2, a Na-Cl cotransporter-like protein, in a Cl uptake mechanism in zebrafish (Danio rerio). Am J Physiol Regul Integr Comp Physiol 296(5), R1650-R1660, 2009.
  48. Tseng, D. Y., Chou, M. Y., Tseng, Y. C., Hsiao, C. D., Huang, C. J., Kaneko, T., and Hwang, P. P. Effects of Stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo. Am J Physiol Regul Integr Comp Physiol 296(3), R549-R557, 2009. 
  49. Tseng, Y. C., Lee, J. R., Chang, J. C. H., Kuo, C. H., Lee, S. J., and Hwang, P. P. Regulation of lactate dehydrogenase in tilapia (Oreochromis mossambicus) gills during acclimation to salinity challenge. Zool Stud 47(4): 473-480, 2008. (Zoology; Ranking: 85/162=52 %; IF: 1.054) 
  50. Tseng, Y.C., and Hwang, P. P. Some insights into energy metabolism for fish osmoregulation. Comp Biochem Physiol C Toxicol Pharmacol 148(4), 419-429, 2008. (Invited review article) 
  51. Chang, J. C. H., Wu, S.M., Tseng, Y. C., Lee, Y. C., Baba, O., and Hwang, P. P. Regulation of glycogen metabolism in gills and liver of the euryhaline tilapia (Oreochromis mossambicus) during acclimation to seawater. J Exp Biol 210(19), 3494-3504, 2007.
  52. Tseng, Y. C., Huang, C. J., Chang, J. C., Teng, W. Y., Baba, O., Fann, M. J., and Hwang, P. P. Glycogen phosphorylase in glycogen-rich cells is involved in the energy supply for ion regulation in fish gill epithelia. Am J Physiol Regul Integr Comp Physiol 293(1), R482-R491, 2007.
  53. Liou, Y. M., Tseng, Y. C., and Cheng, J. C. Spectrofluorometric analysis of length-dependent conformational changes in cardiac troponin C. J Muscle Res Cell Motil 23(4), 309-315, 2002.

(C)Peer-Reviewed Publications (Domestic Journal)

  1. Wang, H. Y., Wang, Y. C., Kuan, P. L., Tseng, Y. C. (2018) Glutamate-glutamine metabolism in euryhaline tilapia (Oreochromis mossambicus) muscle under salinity challenges. J. Taiwan Fish Res, .26(1): 33-45.
  2. Chou, P. H., Tseng, Y. C., Shao, Y. T. (2018) Cellular and physiological explorations of acid-tolerant mechanisms of hydrothermal vent crabs (Xenograpsus testudinatus). J Oce Underwa Technol, 28(3): 10-12.