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研究人員|中央研究院 細胞與個體生物學研究所

研究人員
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側邊選單開關 研究人員
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Dr. Stephan Schneider's Lab

  • 史耐德Stephan Q. Schneider
    助研究員Assistant Research Fellow
    • 專長:Evolutionary Cell & Developmental Biology, Molecular Evolution & Animal Phylogenetics Comparative Genomics
    • 信箱:sqschneider@gate.sinica.edu.tw
    • 電話:02-2789-9512
    • 位置:R145/ICOB
實驗室簡介展開收合

Introduction

Research in the Schneider laboratory aspires to define ancestral core molecular features of developmental and cell biological mechanisms in metazoans. Our unique research focus exploits a powerful emerging model system, the marine annelid, Platynereis dumerilii. Combining experimental embryology, molecular biology and bioinformatics, we target early embryonic development in Platynereis and utilize comparative genome-wide ‘systems’ approaches. Cells in a developing embryo integrate different molecular cues to activate or inhibit transcription in a cascade of events known as gene regulatory networks (GRNs). The long term goal of the Schneider laboratory is to unravel embryonic GRNs in order to reconstruct the core conserved ‘molecular tool kit’ in animal evolution and to delineate mechanisms by which GRNs generate distinct cell fates and cell types. Unraveling the GRNs in Platynereis contributes to a broader scientific understanding of how gene networks drive cell specification, differentiation and morphological diversity, all fundamental questions in developmental biology and animal evolution, a major goal in evolutionary developmental biology.

     Earlier studies on the role of wnt/beta-catenin signaling in animal evolution resulted in the discovery of the nuclear localization of beta-catenin in the organizer of fish and frog embryos (Schneider et al., 1996), and the discovery of a global beta-catenin mediated binary specification module in early Platynereis embryos (Schneider & Bowerman, 2007 & 2013). To gain molecular insight into this intriguing specification module, the Schneider laboratory developed comprehensive resources including the transcriptional blueprint of the early Platynereis embryo (Chou et al., 2016), and the innovative research tool Pdumbase (Chou et al., 2018), which enables unrestricted exploration and dissection of every early biological process. To date, these have allowed us to define the molecular inputs and outputs of the beta-catenin ‘specification’ module (Pruitt et al., 2014, Bastin et al., 2015, Pruitt et al., in preparation), the asymmetric cell division pathways (Nakama et al., 2017), and gene regulatory networks (GRNs) in cell lineages (Chou et al., 2016 & 2018). Current exciting research avenues include the molecular dissection of ‘organizer activity’ in Platynereis (Haillot & Schneider, in preparation), and the comprehensive molecular definition of a differentiated multi-ciliated cell type (Acevedo-Luna & Schneider, in preparation), including GRNs and differentiation gene batteries by beta-catenin-induced cell fate transformation.

Online Resource

  • Chou, H.-C., Acevedo-Luna, N., Kuhlman, J.A., and Schneider, S.Q.
  • PdumBase: A transcriptome database and research tool for Platynereis dumerilii and other metazoans
  • Please use this temporary link to access PdumBase: http://140.109.48.81/platynereis
實驗室成員展開收合
姓名 職稱 電話 Email 備註
史耐德 助研究員 02-2789-9512 sqschneider@gate.sinica.edu.tw
Steffanie Meha 研究助理 02-2789-9573 as0200427@gate.sinica.edu.tw
Grace Sonia 研究助理 02-2789-9573 grace.sonia15@yahoo.co.id
Shianlei Ho 研究助理 02-2789-9573 sherry121@gate.sinica.edu.tw
Yun-Ju Lee 研究助理 02-2789-9573 as0192074@gate.sinica.edu.tw
Wahyu Cristine Apriliasta BR Pinem 博士生 02-2789-9573 christ22yu@gmail.com
著作展開收合
1
Wu, L., Hiebert, L.S., Klann, M., Passamaneck, Y., Bastin, B.R., Schneider, S.Q., Martindale, M.Q., Seaver, E.C., Maslakova, S.A., and Lambert, J.D. (2020) Genes with spiralian-specific protein motifs are expressed in spiralian ciliary bands. Nature Communications, 11:4171, 1-11. DOI: 10.1038/s41467-020-17780-7
2
Bastin, B.R. , and Schneider, S.Q. (2019) Taxon-specific expansion and loss of tektins inform metazoan ciliary diversity. BMC Evolutionary Biology, 19(1):40. 1-25. DOI: 10.1186/s12862-019-1360-0
3
Chou, H.-C. , Acevedo-Luna, N. , Kuhlman, J.A., and Schneider, S.Q. (2018) PdumBase: A transcriptome database and research tool for Platynereis dumerilii and early development of other metazoans. BMC Genomics, 19(1):618. DOI: 10.1186/s12864-018-4987-0
4
Nakama, A., Chou, H.-C., and Schneider, S.Q. (2017) The asymmetric cell division machinery in the spiral-cleaving egg and embryo of the marine annelid Platynereis dumerilii. BMC Developmental Biology, 17:16. 1-22. DOI: 10.1186/s12861-017-0158-9 (BMC series blog: top three articles of 2017)
5
Roy, S., Natakunda, K., Chou, H.-C., Pal, N., Farris, C., Schneider, S.Q., and Kuhlman, J.A. (2017) Defining the transcriptomic landscape of the developing enteric nervous system and its cellular environment. BMC Genomics, 18(1): 290.1-24. DOI: 10.1186/s12864-017-3653-2
6
Chou, H.-C., Pruitt, M.M., Bastin, B.R., and Schneider, S.Q. (2016) A transcriptional blueprint for a spiral-cleaving embryo. BMC Genomics, 17:552.1-25. DOI: 10.1186/s12864-016-2860-6 [reviewed in Blum & Ott (2018) Current Biology, 28]
7
Bastin, B.R., Chou, H.-C., Pruitt, M.M., and Schneider, S.Q. (2015) Structure, phylogeny and expression of the Frizzled related gene family in the lophotrochozoan annelid Platynereis dumerilii. EvoDevo. 6, 37, 1-24. DOI: 10.1186/s13227-015-0032-4
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Pruitt, M.M., Letcher, E.J., Chou, H.-C., Bastin, B.R., and Schneider, S.Q. (2014) Temporal and spatial expression of the wnt gene complement in a spiral-cleaving embryo and trochophore larva. Special Issue ‘Spiralian Model Systems’. Int J Dev Biol. 58(6-7-8): 563-573. DOI: 10.1387/ijdb.140084ss
9
Schneider, S.Q. and Bowerman, B.A. (2013) Animal development: An ancient beta-catenin switch? Current Biology, 23, R313-5. DOI: 10.1016/j.cub.2013.03.011
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Gillis, W.J., St John, J., Bowerman, B.A., and Schneider, S.Q. (2009) Whole genome duplications and expansion of the vertebrate GATA transcription factor gene family. BMC Evolutionary Biology 9, 207, 1-19. DOI: 10.1186/1471-2148-9-207
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Gillis, W.J., Bowerman, B.A., and Schneider, S.Q. (2008) The evolution of protostome GATA Factors: molecular phylogenetics, synteny, and intron/exon structure reveal orthologous relationships. BMC Evolutionary Biology 8, 112, 1-15. DOI: 10.1186/1471-2148-8-112
12
Schneider, S.Q. and Bowerman, B.A. (2007) beta-catenin asymmetries after all animal/vegetal- oriented cell divisions in Platynereis dumerilii mediate binary cell-fate specification. Dev Cell 13, 73-86. (cover photo) DOI: 10.1016/j.devcel.2007.05.002
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Gillis, W.J., Bowerman, B.A., and Schneider, S.Q. (2007) Ectoderm- and endomesoderm- specific GATA transcription factors in the marine polychaete Platynereis dumerilii. Evol & Dev 1, 39-50. DOI: 10.1111/j.1525-142X.2006.00136.x
14
Schneider, S.Q., Finnerty, J.R. and Martindale, M.Q. (2003) Protein Evolution: Structure-Function Relationships of the Oncogene beta-catenin in the Evolution of Multicellular Animals. JEZ (MDE). 295B, 25-44. DOI: 10.1002/jez.b.6
15
Schneider, S.Q. and Bowerman, B. (2003) Cell polarity and the cytoskeleton in the Caenorhabditis elegans zygote. Annu Rev Genet 37, 221-49. DOI: 10.1146/annurev.genet.37.110801.142443
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Kurth, T., Fesenko I., Schneider, S., Münchberg, F., Joos T.O., Spieker, T. and Hausen, P. (1999) Immunocytochemical Studies of the Interactions of Cadherins and Catenins in the early Xenopus Embryo. Dev Dyn 215, 155-169. DOI: 10.1002/(SICI)1097-0177(199906)215:2<155::AID-DVDY8>3.0.CO;2-S
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Schneider, S., Steinbeisser, H., Warga, R.M. and Hausen, P. (1996) Beta-catenin Translocation into Nuclei Demarcates the Dorsalizing Centers in Frog and Fish Embryos. Mech Dev. 57, 191-198. DOI: 10.1016/0925-4773(96)00546-1
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Kurth, T., Schwarz, H., Schneider, S. and Hausen, P. (1996) Fine structural immunocytochemistry of catenins in amphibian and mammalian muscle. Cell Tiss Res. 286, 1-12. DOI: 10.1007/s004410050669
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Müller, H-.A.J., Kühl, M., Finnemann, S., Schneider, S., van der Poel, S.Z., Hausen, P. and Wedlich, D. (1994) Xenopus Maternal Cadherins: A Group of Distinct Members of the Cadherin Family Coexpressed in the Early Embryo. Mech Dev 47, 213-223. DOI: 10.1016/0925-4773(94)90040-x
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Schneider, S., Herrenknecht, K., Butz, S., Kemler, R. and Hausen, P. (1993) Catenins in Xenopus Embryogenesis and their Relation to the Cadherin-Mediated Cell-Cell Adhesion System. Development 118, 629-640.
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Schneider, S., Fischer, A. and Dorresteijn, A.W.C. (1992) A Morphometric Comparison of Dissimilar Early Development in Sibling Species of Platynereis (Annelida, Polychaeta) Roux`s Arch Dev Biol 201, 243-256. DOI: 10.1007/BF00188755