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側邊選單開關 研究人員
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  • Cheng-Fu Kao大頭照
    Cheng-Fu Kao Cheng-Fu Kao老師 Research ID Cheng-Fu Kao老師 ORCID
    Research Fellow & Deputy Director
    • 專長：Chromatin Structure & Dynamics, DNA Replication and Repair
    • 信箱：ckao@gate.sinica.edu.tw
    • 電話：02-2787-1515
    • 網站： Cheng-Fu Kao's Lab
    • 位置：R234/ICOB
友善列印
經歷簡介展開收合
2020-
Research fellow, Institute of Cellular and Organismic Biology, Academia Sinica
2015-2020
Associate research fellow, Institute of Cellular and Organismic Biology, Academia Sinica
2006- 2015
Assistant research fellow, Institute of Cellular and Organismic Biology, Academia Sinica
2005- 2006
Research Faculty, Dept. of Molecular and Microbiology, University of New Mexico
2002- 2005
Postdoctoral Research Fellow, Dept. of Molecular and Microbiology, University of New Mexico
2002
University of Edinburgh Ph. D, Biochemistry
研究方向展開收合

• Chromatin structure and function

• Metabolism on chromatin dynamics and epigenetics

• Chromatin regulatory mechanisms in development and disease

The research in my laboratory centers on the molecular mechanisms that determine Chromatin structure and Cellular functions. The density and positioning of nucleosomes sterically determine the ability of cellular machinery to access the genome. Consequently, chromatin structure has a crucial role in diverse processes including activating or repressing transcription to control functions such as cell fate, the cell cycle, and DNA damage repair. A critical component of chromatin-mediated regulation uses histone post-translational modifications (PTMs), in which histones integrate cellular signals to compose chromatin-dependent functions. Given the regulatory role of chromatin for all DNA-templated processes, it is not surprising that many developmental syndromes are caused by mutations in the protein machinery that ‘writes’, ‘reads’ and ‘erases’ these histone marks and their regulations are frequently altered in cancer, and in many cases these mutations are oncogenic drivers or contributors to tumor progression.

代表著作展開收合
1

Lin CY, Chang YM, Tseng HY, Shih YL, Yeh HH, Liao YR, Hsu CL, Chen CC, Yan YT*, Kao CF*. Epigenetic regulator RNF20 underlies temporal hierarchy of gene expression to regulate postnatal cardiomyocyte polarization. Cell Rep. 2023 Nov 28;42(11):113416. doi: 10.1016/j.celrep.2023.113416.

2

Huang JH, Liao YR, Lin TC, Tsai CH, Lai WY, Chou YK, Leu JY, Tsai HK*, Kao CF*. iTARGEX analysis of yeast deletome reveals novel regulators of transcriptional buffering in S phase and protein turnover. Nucleic Acids Res. 2021 Jul 21;49(13):7318-7329. doi: 10.1093/nar/gkab555.

3

Hsu CL, Chong SY, Lin CY, Kao CF*. Histone dynamics during DNA replication stress. J Biomed Sci. 2021 Jun 19;28(1):48. doi: 10.1186/s12929-021-00743-5.

4

Hsu CL, Lo YC, Kao CF*. H3K4 Methylation in Aging and Metabolism. Epigenomes. 2021 Jun 18;5(2):14. doi: 10.3390/epigenomes5020014.

5

Chong SY, Cutler S, Lin JJ, Tsai CH, Tsai HK, Biggins S, Tsukiyama T, Lo YC and Kao CF*. H3K4 methylation at active genes mitigates transcription-replication conflicts during replication stress. Nat Commun. 2020 Feb 10;11(1):809. doi: 10.1038/s41467-020-14595-4

6

Wu MY, Lin CY, Tseng HY, Hsu FM, Chen PY and Kao CF*. H2B ubiquitylation and the Asf1 histone chaperone mediate the formation and maintenance of heterochromatin architecture. Nucleic Acids Res. 2017 May 17. doi: 10.1093/nar/gkx422. (*correspondent author)

7

Hung SH, Wong RP, Ulrich HD* and Kao CF*. Bre1-mediated mono-ubiquitylation of H2B contributes to the bypass of DNA damage during and after DNA replication Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2205-E2214. (*correspondent authors)

8

Hsu HE, Liu TN, Yeh CS, Chang TH, Lo YC*, Kao CF*. Feedback Control of Snf1 Protein and Its Phosphorylation Is Necessary for Adaptation to Environmental Stress. J Biol Chem. 2015 Jul 3;290(27):16786-96. doi: 10.1074/jbc.M115.639443.

9

Lin CY, Wu MY, Gay S, Marjavaara L, Lai MS, Hsiao WC, Hung SH, Tseng HY, Wright DE, Wang CY, Hsu GSW, Devys D, Chabes A and Kao CF*. (2014) H2B mono-ubiquitylation facilitates fork stalling and recovery during replication stress by coordinating Rad53 activation and chromatin assembly. PLoS Genet 10: e1004667. ( *Correspondent author)

10

Bonnet J*, Wang CY*, Baptista T, Vincent SD, Hsiao WC, Stierle M, Kao CF, Tora L& and Devys D& (2014) The SAGA coactivator complex acts on the whole transcribed genome and is required for RNA polymerase II transcription. Genes Dev. 2014 Sep 15;28(18):1999-2012. doi: 10.1101/gad.250225.114. ( *First authors; &Correspondent authors)